C Ago2 RNA immunoprecipitation (RIP) was performed, followed by qRT–PCR. A RIP assay with an Ago2 antibody was used to assess the binding ability between miR-144/451 and Ago2 mRNA in wt MEFs, TAp63−/− MEFs, or TAp63−/− MEFs transfected with a TAp63 expression construct. ...
2a). The RIP assay result showed that serum stimulation greatly increased the association between pre-miR-19b1-WT with AGO2, but not the interactions between three mutants pre-miR-19b1-UGAUUG, -AUAUUG and -AUAUAU with AGO2 (Fig. 5h). This result suggested that the UGUGUG motif of ...
RIPAb+ Ago2 - RIP Validated Antibody and Primer Set Promotional Text: Special Shipping Offer on Antibodies 100% Performance Guaranteed Trade Name: Upstate (Millipore) Product Overview: RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody...
Activity of the FNIP1 and TOB1 reporters in this assay was inconsistent with such a model (Fig. S9): PUM effects for TOB1 were not significant, while FNIP1 showed increased repression for the mutant site construct that did not reach statistical significance, and a further, significant ...
The proliferation and differentiation of mammalian skeletal muscle satellite cells (MuSCs) are highly complicated. Apart from the regulatory signaling cascade driven by the protein-coding genes, non-coding RNAs such as microRNAs (miRNA) and circular RNAs
RNA immunoprecipitation assay. RIP assay was carried out following the method described by Christoph Ufer42 with some modifications. Please refer the supplementary method for details. Scientific Reports | 5:15793 | DOI: 10.1038/srep15793 13 www.nature.com/scientificreports/ Assessing the ...