Microarray expression analyses following miRNA transfection/inhibition and, more recently, Argonaute cross-linked immunoprecipitation (CLIP)-seq assays have been used to detect miRNA target sites. CLIP and expression approaches measure differing stages of miRNA functioning-initial binding of the miRNP comple...
第二个模型能解释一些用CLIP方法在mRNA上发现的一些莫名其妙出现的孤儿结合位点(orphan binding sites)。第二个模型中是否还需要小RNA与mRNA结合尚不清楚。图自[5] 人类细胞中的四种Ago: 由于四种Ago蛋白都能与小RNA结合、发挥作用,最初人们认为这四种Ago蛋白在功能上是冗余的。但实际并非如此,在许多严重的神经疾病...