AgotronArgonauteCLIPGene regulationMirtronRNA sequencingSmall RNA profilingmiRNATimely and accurate regulation of gene expression is essential to all organisms. MicroRNAs (miRNAs) are a well-characterized and important class of gene expression regulators. We recently identified......
small_RNA_seq_counts_7D.txt Input table for Figure 7D Repository files navigation README AgoCLIP_2020 Code used for the manuscript: "Argonaute-CLIP delineates versatile, functional RNAi networks in Aedes aegypti, a major vector of human viruses"...
Using AGO2 RIP-seq and PAR-CLIP-seq we show that the DNA-damage-induced increase in binding of let-7 family members to the RISC complex is functional. We unambiguously determine the global miRNA-mRNA interaction networks involved in the DNA damage response, validating them through the ...
Here, we profiled the overall changes in Ago2-mRNA interactions upon arsenite stress by cross-linking immunoprecipitation (CLIP) followed by high-throughput sequencing (CLIP-seq). Ago2 displayed a significant remodeling of its transcript occupancy, with the majority of 39 untranslated region (UTR) ...
Transcriptome-wide changes in AGO2-mRNA binding upon PUM knockdown were quantified by CLIP-seq, and the presence of PUM binding on the same 3′UTR corresponded with cooperative and antagonistic effects on AGO2 occupancy. In addition, PUM binding sites that overlap with AGO2 showed differential, ...
RNA-seq and cross-linking immunoprecipitation (CLIP)-based techniques from ENCODE and FANTOM consortia. We show that 3′UTR-derived RNAs present biochemical properties similar to their 5′ capped counterparts and that they can be as abundant as the protein-coding version of the host transcript, or...
starBase: a database for exploring microRNA–mRNA interaction maps from Argonaute CLIP-Seq and Degradome-Seq data MicroRNAs (miRNAs) represent an important class of small non-coding RNAs (sRNAs) that regulate gene expression by targeting messenger RNAs. However, assign... JH Yang,JH Li,S Peng...
and atherosclerosis14,15(Supplementary Fig.6eand f). As AMPK is known as a critical regulator of energy metabolism, we further assessed the role of Ago2 inAmpka1expression. Analyzing a public database of photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation (PAR-CLIP) with Ago...
ApplicationsSuggested dilutionResults IP (RIP) 8μg per IP Fig 1 HITS-CLIP - Fig 2 WB 1:500 Fig 3 IF 1:500 Fig 4 IHC 1:500 Validation Data Figure 1. Immunoprecipitation of endogenous Argonaute proteins using the Diagenode Ago monoclonal antibody Hela cells (10 cm dish per IP) were subj...
Cattle silhouette from clipartkid. Caribou silhouette by mystica/CC. dog and the low concentration of other vertebrate DNA prevented a meaningful comparison of abundances in this layer with the biomass estimates from zooarchaeological analyses. We found the midden samples from the Norse settlement of...