Afterwards, proteins were transferred from gels into the nitrocellulose membranes (Bio-Rad, Hercules, CA, USA) using the Trans-Blot Turbo Transfer System (Bio-Rad, Hercules, CA, USA). Following the transfer, total proteins on the membranes were detected using the ChemiDoc imager. After imaging...
Technical duplicates containing 10 ng of reverse transcribed RNA were amplified with the GoTAQ Probe qPCR Master Mix (Promega) using a two-step cycling protocol (95 °C for 15 s, 60 °C for 60 s; 40 cycles, Bio-Rad CFX 96 Cycler). The following gene expression assays were ...
Keywords: spatial accessibility; aging services; residential aged care facilities (RACFs); Wuhan 1. Introduction In 1982, the Vienna World Assembly on Aging acknowledged that when the senior population proportion (aged 65 and above) is more than 7%, the country or region would enter a seriously...
Baluchnejadmojarad, T.; Rabiee, N.; Zabihnejad, S.; Roghani, M. Ellagic Acid Exerts Protective Effect in Intrastriatal 6-Hydroxydopamine Rat Model of Parkinson’s Disease: Possible Involvement of ERβ/Nrf2/HO-1 Signaling. Brain Res. 2017, 1662, 23–30. [Google Scholar] [CrossRef] Fu...
This oxidase is a multi-sub unit enzyme composed of a membrane-bound heterodimeric subunit (p22phox and gp91phox) and several cytoplasmic regulatory sub units including Rac1, p47phox, and p67phox. NADPH oxidases are crucial regulators of redox signaling through tightly regulated production of low...
Viability of magnetically captured cells (eluted into a new tube away from the magnet) was assessed by trypan blue staining (1:2 dilution sample in trypan blue; Cat#1450013; Bio Rad, Hercules, CA USA). Cell viability was quantified as the proportion of cells that did not take up the ...
Western blotting: Protein concentrations were determined using a DC protein assay (Bio-Rad) and aliquots of samples were normalized to 0.2 μg/μL. Laemmli sample buffer (final concentration: 6.25 mM Tris, pH 6.8, 2% SDS, 10% glycerol, 1.25% 2-mercaptoethanol, 0.1% bromophenol blue) was ad...
Proteins were transferred to a PVDF membrane, using the Trans-Blot Turbo™ Transfer System (Bio-Rad, Hongkong, China) and blocked in 5% BSA/Tris Buffer Saline/0.1% Tween 20 (TBST) for 2 h at room temperature, followed by overnight incubation at 4 ˝C with gentle agitation with ...