A recent technical report inNature Cell Biologyhas introduced a novel method to examine specific alterations inDNAfollowing replication. Scientists have devised a highly sensitive, quantitative method utilizing mass spectrometry known as iDEMS (isolation of DNA by EdU labeling for Mass Spectrometry). “...
Homologous recombination (HR) plays critical roles in repairing lesions that arise during DNA replication and is thus essential for viability. RAD51 plays important roles during replication and HR, however, how RAD51 is regulated downstream of nucleofilament formation and how the varied RAD51 function...
a process known as chromatin domain inheritance (CDI). CDI is of necessity linked to DNA replication, DNA repair, and transcription, but must be considered in any nucleosomal histone turnover model. Because it is hard
M protein has an extended N-terminal loop, an amphipathic perimembrane helix (M-H), and two transmembrane helices (M-T1 and M-T2)35,36. Unlike the immature virus, the E stem region of the mature virus harbors two lipid-like ligand binding sites: the S1 site between E-H1 and E-H2 ...
The decrease in polarity of DNA then promotes enrichment in the organic, non-polar phase. RNA however has an additional negative charge due to its 2’OH group with a pKa of 13.0. Additionally and unlike in DNA, the nucleobases are not all paired via H-bonds in a double helix, meaning ...
While MDF-1 prevents both loss and gain of whole chromosomes during mitosis, DOG-1 prevents seg- mental aneuploidies by ensuring proper replication of guanine(G)-rich DNA [4-6]. G-rich DNA can adopt a four-stranded helical G-quadruplex (G4) DNA struc- ture [7-9], which can pose a...
The cloned antisense fragments can be cut out from the cloning vector and inserted into an expression vector. An appropriate expression vector generally includes (1) a prokaryotic DNA factor which encodes an origin of replication in bacterial hosts and an antibiotics resistant marker, (2) a factor...
The myxovirus resistance (Mx) proteins belong to interferon-induced dynamin GTPase and play pivotal role in the inhibition of replication of numerous viruses. These antiviral proteins are released in usual or diseased condition to prevent the viral attac
Tissue CulturesA Vertebrate Model for Human Molecular GeneticsABC Transporters: From Basic Functions to DiseasesActin-Myosin Cytoskeleton Regulation and Function Series 2Adaptation of Oral Tissue and Bone Remodeling to Systemic ImbalancesAdding New Pieces in the Osteopontin PuzzleAdenosine and Adenosine ...
antibiotics Article Protein Expression Modifications in Phage-Resistant Mutants of Aeromonas salmonicida after AS-A Phage Treatment Catarina Moreirinha 1,† ID , Nádia Osório 2,†, Carla Pereira 1,†, Sara Simões 2, Ivonne Delgadillo 3 and Adelaide Almeida 1,* ID 1 Departament of ...