LOND. ASSISTANT PHYSICIAN AND PATHOLOGIST LATE CHLOROFORMIST, PADDINGTON-GREEN CHILDREN S HOSPITAL ; ASSISTANT PHYSICIAN, NORTH-WEST LONDON HOSPITAL ; PHYSICIAN, REGENT S-PARK HOSPITAL FOR EPILEPSY AND PARALYSIS.Guthrie, L. G.: On some fatal after‑effects of chloroform on ...
RNA was isolated by adding 200 µl of chloroform (Sigma-Aldrich, catalogue no. 288306) to samples, followed by shaking and centrifugation at 4 °C, 12,000g for 15 min. The aqueous phase was transferred into a new tube and RNA was isolated and DNase treated with the RNA Clean ...
AMPUTATION AT THE SHOULDER-JOINT UNDER THE INFLUENCE OF CHLOROFORM IN A CASE OF GUN-SHOT WOUND A study of the effects of amputating the primary seminal roots of Elymus junceus and Agropyron intermedium indicates that the primary seminal roots can be amputated without affecting the survival of the...
Here, we investigate the effects of conservation agriculture compared to conventional agriculture on 17 soil properties, microbial diversity and crop yields, during eight-years’ experimental warming. An overall positive effect of warming on soil health over time under conservation agriculture is ...
fibrillation resulting from topical application of aconitine in the anesthetized dog and ventricular fibrillation induced by chloroform asphyxiation in the mouse... AW Gomoll,JE Byrne,MJ Antonaccio - 《American Journal of Cardiology》 被引量: 19发表: 1986年 Risk Factor Analyses for the Return of Sp...
Briefly, DRGs and spinal cord segments at the L1-L6 level, from both the sham and SCI groups, were homogenized and added to internal standards (AEA-d4, Sigma, USA) containing 2.0 mL of methanol. The supernatants were then extracted with chloroform and centrifuged at 3000 rpm for 10 min ...
Here, we investigate the effects of conservation agriculture compared to conventional agriculture on 17 soil properties, microbial diversity and crop yields, during eight-years’ experimental warming. An overall positive effect of warming on soil health over time under conservation agriculture is ...
RNA preparation and gene expression analysis of pro-inflammatory cytokines by quantitative real-time polymerase chain reaction (qPCR) For total RNA isolation and PCR, RNA was isolated with TRIZOL reagent (Invitrogen, Carlsbad, CA, USA) as recommended by the manufacturer, followed by chloroform extract...
(AEA-d4, Sigma, USA) containing 2.0 mL of methanol. The supernatants were then extracted with chloroform and centrifuged at 3000 rpm for 10 min at 4 °C. The organic phases were dried under a stream of nitrogen, and the residues were re-dissolved in 2 mL of methanol. All samples were...
(Christ, BETA 2-8 LSC plus, PRAGOLAB s. r. o., Slovakia). The crude cell walls were rid of long fatty acids in the Soxhlet apparatus using chloroform/methanol (ratio 2:1, 4 h). The cleaned cell wall material was left to dry at room temperature, suspended in 50 mM phosphate ...