We covalently coupled purified BC2-Nb to Sepharose beads and generated an affinity matrix that we refer to as BC2 nanotrap. First, we tested the ability of the BC2 nanotrap to precipitate BC2T-tagged proteins directly from crude lysates. Therefore, the soluble protein frac- wtioitnhstohfeEB....
Ultimately, the 11 muteins successfully purified were used to investigate the Ala substitution effects (Figure 1C). Results of the ITC experiments are given in Table 1. The dissociation constant (KD) of the scFv(WT) for Ig5 was 239 ± 4 nM. The mutein Y104HA showed about 10-fold lower...
PAS-1, a protein affinity purified from Ascaris suum worms, maintains the ability to modulate the immune response to a bystander antigen [J]. Immunol Cell Biol, 2006, 84(2): 138-44.Oshiro TM,Enobe CS,Ara jo CA. PAS-1,a protein affinity purified from Ascaris suum worms,maintains the ...
Produced in rabbits immunized with purified, recombinant Human NKG2A / NKG2 / CD159A / KLRC1 (rh NKG2A / NKG2 / CD159A / KLRC1; Catalog#13905-H07H; NP_002250; Arg100- Leu233). NKG2A / NKG2 / CD159A / KLRC1 specific IgG was purified by Human NKG2A / NKG2 / CD159A / KLRC1 ...
As of June 2021, S proteins have exclusively been purified using difficult-to-scale, low-yield methodologies such as affinity and size-exclusion chromatography. Herein we present the first known non-affinity purification method for two S constructs, S_dF_2P and HexaPro, expressed in the ...
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific). Storage: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receip...
CA protein was purified under denaturing conditions using gel-filtration chromatography followed by refolding. All proteins were obtained at a purity >98%. Both aforementioned C-terminal extensions led to dramatic changes in behavior of the products and they also affected the tendency to form ...
Our AP-MS protocol consisted in the following steps: A) The protein of interest (PARP-1, PARP-2, or PARG) was purified from a human cell lysate together with its binding partners. B) Proteins in the pulled-down complexes were separated by SDS-PAGE and then proteolyzed with trypsin. C...
In agreement with this we have found here that the binding affinity for 3H-gabapentin is increased in purified DRM fractions from brain. Gabapentin interacts with a binding site found upstream of the VWA domain3,24,34. In agreement winitbhotthhisα, 2wδ e-1haanvde fαo 2uδn...
Supernatants were harvested after 96 hours of protein expression and purified over HiTrap Protein A columns (GE Healthcare). Crystallization and Structural Determination of FD6:CD47 Complex: E. coli derived FD6 and deglycosylated, insect-derived CD47 were mixed at a 1:1 ratio and treated with ...