In vitro affinity maturation of therapeutic monoclonal antibodies is commonly applied to achieve desired properties, such as improved binding kinetics and affinity. Currently there are no universally accepted protocols for generation of variegated antibody libraries or selection thereof. Here, we performed ...
Mimicking Somatic Hypermutation: Affinity Maturation of Antibodies Displayed on Bacteriophage Using a Bacterial Mutator Strain Human antibodies can now be isolated from antibody repertoires displayed on the surface of filamentous bacteriophage in a process that mimics the primary i... NM Low,P Holliger,...
文献阅读——Affinity maturation of SARS-CoV-2 neutralizing antibodies confers potency, breadth ... goblin7 20508 目录 收起 1. Author 2. Background 2.1 体细胞突变(somatic mutation) 2.2 RBD抗体的分类 3. Methods 4. Results 5. Discussion 1. Author Paul D. Bieniasz Paul Bieniasz试图探寻宿主...
Affinity maturation of antibodies is achieved by antigen selection of specific B lymphocyte clones expressing membrane immunoglobulin (mIg). A model system was generated to examine antigen:mIg interactions by expressing mIg specific for a hapten, phosphocholine (PC). The murine B lymphoma M12.4 was ...
In vivo affinity maturation of antibodies involves mutation of hot spots in the DNA encoding the variable regions. We have used this information to develop a strategy to improve antibody affinity in vitro using phage display technology. In our experiment with the antimesothelin scFv, SS(scFv), ...
文献阅读009——Affinity maturation of SARS-CoV-2 neutralizing antibodies confers potency, breadth, and ... 1. Author Paul D. Bieniasz Paul Bieniasz试图探寻宿主基因和途径如何影响逆转录病毒的复制,比如HIV-1。Bieniasz和他的团队主要关注人类和灵长类免疫缺陷病毒,致力于确定病毒基因和蛋白质的功能,并...
Moreover, the affinity-matured antibodies retained high conformational specificity for Aβ aggregates, as observed for aducanumab and unlike crenezumab. Notably, the affinity-maturated antibodies displayed extremely low levels of nonspecific interactions, as observed for crenezumab and unlike aducanumab....
2006. Evaluation of molecular models for the affinity maturation of antibodies: roles of cytosine deamination by AID and DNA repair. Chem. Rev. 106: 700 -719.Samaranayake M, Bujnicki JM, Carpenter M, Bhagwat AS. 2006. Eval- uation of molecular models for the affinity maturation of ...
Efficient affinity maturation of antibodies in an engineered chicken B cell line DT40-SW by increasing point mutation(CELL AND TISSUE ENGINEERING) 2010. Efficient affinity maturation of antibodies in an engineered chicken B cell line DT40-SW by increasing point mutation. J. Biosci. Bioeng. 110:...
VL, variable region of an antibody light chain FACS, fluorescent-activated cell sorting LC, light chain HC, heavy chain 1. Introduction The past decade has seen recombinant antibodies become an increasingly important group of drugs, which currently represent over 30% of biopharmaceuticals in clinical...