We observed loss of cell–cell junctions and protrusion development 4–12 h after adhesion induction in vivo (Fig. 2f and Supplementary Video 5). Consequently, 5 days after adhesion induction, GFP+ mesothelial cells were seen between the fused parietal and visceral layers, with a host of ...
macrophages to participate in the cell barrier formation in the “repeated ischemic button creation model” (Supplementary Fig.10a, b). In this model, we created an additional ischemic button on the right-side peritoneal wall on day 3 after the first ischemic button creation on the left-side p...
The number of focal adhesions was determined in 120 × 120 μm area. The threshold was set between 1.5 and 2.0% (in the case of pFAK antibody, rabbit host) or 3.5 and 4.0 (in the case of pFAK antibody, mouse host) and the image was converted to a binary one. As a result, the...