Addgene, the nonprofit plasmid repository, accelerates science by sharing useful plasmid-based research materials, viral preps, and information worldwide.
1. 了解质粒包含的元件 当今科学家使用的质粒有多种大小,功能差异很大。在最简单的形式中,质粒需要细菌复制起点 (ori)、抗生素抗性基因和至少一个独特的限制酶识别位点。这些元件允许质粒在细菌内繁殖,同时允许对任何不携带质粒的细菌进行选择。此外,限制酶位点允许将待研究的 DNA 片段克隆到质粒中。 Origin of Repli...
获取 NGS 的方法有多种,但最常用的方法之一是 Illumina NGS 平台。这是 Addgene 的测序合作伙伴 seqWell(https://blog.addgene.org/the-power-behind-ngs-plasmid-validation-seqwell?_ga=2.224584666.1761310573.1650342339-1554568351.1648354532)使用的平台。 Illumina NGS 的实际过程与 Sanger 测序没有太大区别。该...
Addgene p53 shRNA plasmid pLVTH-sip53 was used to perform cell transfection in order to study study the regulation mechanism of protein quality control by UBE4B and LSD1. Addgene pLVTHM was used to perform DNA cloning in order to study the regulation of FGF14 on resurgent sodium current in...
Addgene - Plasmid Sequence Analysis INSIDE THE VIDEO Tips on verifying your plasmids including designing primers for sequence analysis and visualizing your results. LENGTH 6 Minutes Addgene - Annealed Oligo Cloning INSIDE THE VIDEO Watch this video to learn how to add new restriction sites to the...
和元生物可以提供从病毒包装(腺相关病毒/慢病毒/腺病毒/逆转录病毒)、质粒构建(过表达/干扰)、细胞稳定株构建、细胞功能学实验、双萤光素酶检测、动物模型构建到药物代谢动力学研究及外泌体研究等热点方向的整体或灵活定制化CRO服务。 此价格为该类型产品标准服务价格, ...
In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA.EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not m...
Plasmid 22924 Standard format: Plasmid sent in bacteria as agar stab 1 $75 Add to Cart This material is available to academics and nonprofits only. Backbone Vector backbonepAAV (Search Vector Database) Backbone sizew/o insert(bp)4000 Vector typeMammalian Expression, AAV Growth in Bacteria Bacter...
英文名称: plasmid 保质期: 3个月/12个月 保存条件: -20℃/-80℃ 上海禾午生物科技有限公司可提供大肠表达质粒、大肠编辑质粒、哺乳编辑质粒、ORF表达质粒、枯草游离质粒、哺乳荧光质粒、哺乳表达质粒、哺乳报告质粒、酵母杂交质粒、腺相关类质粒、慢病毒类质粒、植物表达质粒、大肠克隆质粒、昆虫病毒质粒、蛋白...
(产品信息仅供参考,请与客服确认实际情况后再下单) 货品编号:addgene.68797 数量:-+支 正品货源 质量保障 商品详情 商品评论 商品咨询 品牌:addgene 所属分类:一抗 货品编号: addgene.68797 品牌:addgene 品名:Standard format: Plasmid sent in bacteria as agar stab ...