1. 了解质粒包含的元件 当今科学家使用的质粒有多种大小,功能差异很大。在最简单的形式中,质粒需要细菌复制起点 (ori)、抗生素抗性基因和至少一个独特的限制酶识别位点。这些元件允许质粒在细菌内繁殖,同时允许对任何不携带质粒的细菌进行选择。此外,限制酶位点允许将待研究的 DNA 片段克隆到质粒中。 Origin of Repli...
获取 NGS 的方法有多种,但最常用的方法之一是 Illumina NGS 平台。这是 Addgene 的测序合作伙伴 seqWell(https://blog.addgene.org/the-power-behind-ngs-plasmid-validation-seqwell?_ga=2.224584666.1761310573.1650342339-1554568351.1648354532)使用的平台。 Illumina NGS 的实际过程与 Sanger 测序没有太大区别。该...
Addgene, the nonprofit plasmid repository, accelerates science by sharing useful plasmid-based research materials, viral preps, and information worldwide.
pC1-HyPer-3 (Plasmid #42131) Insert:HYPER 3Synthetic Tags: None Expression: Mammalian Mutation: None Use: Deposited by: Vsevolod Belousov AAVS1 SA-2A-puro-pA donor (Plasmid #22075) PurposeAAV zinc finger donor vector for genomic targeting Insert:SA-PuroHuman Tags: None Expression: Mutation:...
Addgene The nonprofit plasmid repository Verified 15followers Watertown, MA, US https://www.addgene.org Overview Repositories5 Packages People2 More Popular repositoriesLoading giraffegiraffePublic archive JavaScript106 openbioopenbioPublic A collection of open source bioinformatics tools developed and used...
pPAGFP-C1 (Plasmid #11910) Insert: PAGFP Aequorea victoria Tags: None Expression: Mammalian Mutation: These plasmids are essentiall... Use:Deposited by: Jennifer Lippincott-Schwartz pPAGFP-N1 (Plasmid #11909) Insert: pPAGFP-N1 Aequorea victoria Tags: None Expression: Mammalian Mutation: These pl...
plasmid copy : Eric Lander, David Sabatini (backbone, pCW-Cas9, #50661), Feng Zhang (insert, lentiCas9-Blast #52962). resistance markers : 3055 tags : High Copy terms : Blasticidin more info or order: Addgene product webpage company information ...
Ishibashi R, Maki R, Kitano S, Miyachi H, Toyoshima F. Development of an in vivo cleavable donor plasmid for targeted transgene integration by CRISPR-Cas9 and CRISPR-Cas12a. Sci Rep. 2022;12:17775pubmedpublisher Lansing F, Mukhametzyanova L, Rojo Romanos T, Iwasawa K, Kimura M, Paszkows...
HH-CAS-101p414-TEF1p-Cas9-CYC1t(Plasmid #43802) HH-CAS-100pRPR1_gRNA_handle_RPR1t (Plasmid #49014) HH-ZW-041pYLCRISPR Cas9P35s-N HH-ZW-040pYLsgRNA-OsU6a/LacZ(Addgene 66195) HH-ZW-039pYLsgRNA-OsU3(Plasmid 66193) HH-CAS-130pAc-sgRNA-Cas9(addgene 49330) HH-CAS-129pEM-Cas9HF...
HH-CAS-106pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP (Plasmid #71237) HH-CAS-102pCS2-nCas9n-nanos 3'UTR(Plasmid #62542) HH-CAS-103pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HANLS(s)(Plasmid #47868) HH-CAS-102pCS2-nCas9n-nanos 3'UTR(Plasmid #62542) HH-CAS-101p414-TEF1p-Cas9...