With our robust PBMC-Mediated ADCC Assay, researchers and pharmaceutical companies can gain critical insights into the efficacy and safety profile of their antibody candidates, facilitating the development of more effective treatments. Short Protocol ...
询价 索取Protocol 产品描述 产品数据库I. Background 抗体依赖的细胞介导的细胞毒性作用(ADCC,antibody-dependent cell-mediated cytotoxicity )是指抗体的 Fab 段结合病毒感染的细胞或肿瘤细胞的抗原表位,其 Fc 段与杀 伤细胞(NK 细胞、巨噬细胞等)表面的 FcR 结合,介导杀伤细胞直接杀伤靶细胞,它是 抗肿瘤的...
ADCC assay performance and reproducibility using ready-to-use, cryopreserved NK effector cells, A549 target cells expressing a luciferase reporter gene, and anti-EGFR antibody. ADCC Protocol using BioIVT Cryopreserved NK Effector Cells Step-by-step protocol for ADCC assays using cryopreserved NK effector...
Competitive ELISA assay was used to evaluate the ability of ATG-034-S3 to block the interaction of LILRB4 with its ligand ApoE. ADCC assay was PBMC-mediated cytotoxicity and measured by FACS analysis. ADCP assay was M2 macrophage-mediated phagocytosis and measured using FACS analysis. T cell ...
pp 179–192 Cite this protocol Natural Killer Cell Protocols Bice Perussia& Matthew J. Loza Part of the book series:Methods in Molecular Biology((MIMB,volume 121)) 3056Accesses Abstract Crosslinking of molecules involved in the recognition of target cells elicits natural killer (NK) cell cytotox...
IC50 a(μngd/mICl5)0wvaaslutehsewceornecceanltcrualtaiotendabt ywthhiechso5f0tw%aoref Antibody-dependent cell-mediated cytotoxicity assay (ADCC). The protocol is a modified version of that published previously19. For bioluminescence-based assay by luciferase, 50,000 CEM CD4+ target (...
a Real-time impedance-based assay of BRCA WT prostate carcinoma (DU145) cells in the presence or absence of olaparib (ola), human NK cells, and mAbs. b Lysis of BRCA WT cells with or without olaparib pretreatment and NK cells, and with cetuximab (cet) or isotype control at 12 h ...
Transfected cells were first stimulated with 400 μM H2O2 for 24 h, and an apoptosis assay was then performed using Annexin V-FITC/PI Apoptosis Detection Kit (Vazyme, China) according to the manufacturer’s instructions. The cells were assessed by flow cytometry using a BD FACS Aria-IIu...
Freshly isolated peripheral blood mononuclear cells were added to HT-29 target cells with the respective effector:target ratios in a chromium release assay to quantitate NK cell activity as indicated. Donors were chosen with naturally different levels of circulating NK cells; (a) 3.8%, (b) 6.4%...
1 The abbreviations used are: FcRn, neonatal Fc receptor; ADCC, antibody-dependent cellular cytotoxicity; CHO, Chinese hamster ovary; DHFR, dihydrofolate reductase; ELISA, enzyme-linked immunosorbent assay; mAb, monoclonal antibody; NK, natural killer cell; PBMC, peripheral blood monocytic cell. This...