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P38, phospho-P38, ERK, phospho-ERK, JNK, phospho-JNK, caspase 3, cleaved caspase 3, BCL-2, IRF1 and RIG-I were purchased from Cell Signaling Technology; ADAR1 antibody was purchased from Santa Cruz (cat# SC73408, clone 15.8.6). Secondary antibodies were purchased from Promega. Lactate ...
anti-ADAR1 Santa Cruz Biotechnology Cat# sc-73408; RRID: AB_2222767 anti-ZBP1 AdipoGen Cat# AG-20B-0010; RRID: AB_2490191 anti-RIPK3 Cell Signaling Technology Cat# 95702; RRID: AB_2721823 anti-pSTAT1 Cell Signaling Technology Cat# 7649; RRID: AB_10950970 anti-tSTAT1 Cell Signaling Tech...
ADAR1 was detected with ADAR1 antibody clone 15.8.6 (Santa Cruz sc-73408) at 1:1000 dilution. RNA sample preparation and qRT-PCR RNA isolation was performed with RNeasy Plus Mini Kit (Qiagen Cat # 74,134) following the manufacturer’s instructions. Quantitative RT-PCR was performed using ...
The resuspended nuclei were homogenized and centrifuged at 13,000 rpm for 10 min, and the supernatant was then incubated with anti-ADAR1 antibody (sc-73408; Santa Cruz Biotechnology) or mouse IgG (Fujifilm Wako Pure Chemical) for 2 h at 4 °C with gentle rotation, followed by incubation ...
Mutations of theADAR1gene encoding an RNA deaminase cause severe diseases associated with chronic activation of type I interferon (IFN) responses, including Aicardi–Goutières syndrome and bilateral striatal necrosis1,2,3. The IFN-inducible p150 isoform of ADAR1 contains a Zα domain that recognizes...
(Thermo Scientific). The antibodies used were as follows: anti-ADAR1 (Santa Cruz: sc-73408), anti-actin (Abcam: Ab156302). For ADAR1, the antibody was diluted to 1:2000. For Actin, the antibody was diluted to 1:10,000. The uncropped western blot gel is found in Supplementary Fig.7...
the brain tissues was detected by Western blot as described previously [31]. In brief, 30 μg of protein extract was loaded to each lane and separated on 8% polyacrylamide gel with 0.1% SDS. ADAR1 was detected with ADAR1 antibody clone 15.8.6 (Santa Cruz sc-73408) at 1:1000 dilution...
货号:sc-73408 品牌:SantaCruz ADAR1 (D7E2M) Rabbit mAb 价格: 价格电议 产品详情:温馨提示:优宁维所有产品和服务仅用于科学研究,不用于临床应用及其他用途提供产品和服务(也不为任何个人提供产品和服务)! ... 纯度:见优宁维官网% 包装规格:见优宁维官网 用途:详见说明书 用途:详见说明书 货号:14175 品牌:CS...
Mouse monoclonal anti-Adar1 (15.8.6) Santa Cruz Biotechnology Cat#sc-73408; RRID: AB_2222767 Rabbit polyclonal anti-ADAR1-p150 Synaptic Systems GmbH Cat#293003; RRID: AB_2620031 Mouse monoclonal anti-ZBP1 (Zippy-1) Adipogen Cat#AG-20B-0010; RRID: AB_2490191 Mouse monoclonal anti-Histone ...