The quorum-quenching N-acyl homoserine lactone acylase PvdQ is an Ntn-hydrolase with an unusual substrate-binding pocketdoi:10.1073/PNAS.0911839107Marcel BokhovePol Nadal JimenezWim J. QuaxBauke W. DijkstraNational Academy of Sciences
The Pseudomonas aeruginosa PAO1 genome has at least two genes, pvdQ and quiP, encoding acylhomoserine lactone (AHL) acylases. Two additional genes, pa1893 and pa0305, have been predicted to encode penicillin acylase proteins, but have not been characterized. Initial studies on a pa0305 transpos...
Luo,Z.-Q. - 《Journal of Biological Chemistry》 被引量: 207发表: 2000年 The quorum-quenching N-acyl homoserine lactone acylase PvdQ is an Ntn-hydrolase with an unusual substrate-binding pocket. In many Gram-negative pathogens, their virulent behavior is regulated by quorum sensing, in which...
acylases (EC 3.5.1.–) and oxidoreductases (EC 1.1.–) (Fig.1). For each of the main enzyme classes two subclasses of enzymes are known. While a few oxidoreductases have been reported the best studied group of enzymes processing AHL molecules are hydrolases, including lactonase...
However, pvdQ knockout mutants of P. aeruginosa were still able to grow by utilizing 3OC12HSL. To our knowledge, this is the first report of the degradation of AHLs by pseudomonads or other 纬-Proteobacteria, of AHL acylase activity in a quorum-sensing bacterium, of HSL lactonase activity...
of the lactone ring by AHL lactonases results from the addition of a water molecule into AHLs to produce acylhomoserine [4,5,6,7], and in this research the UPLC-MS was used to confirm the AHL degradation activity of RmmL via a lactonase mechanism. The mass spectrum of the degraded pr...
Ddrougnst2h0e18t,y1p6,exoFfOrReaPcEtEioRnRcEaVtIaElWyzed: AHL lactonases (lactone hydrolysis), AHL acylases (cleav2agoef o11f a(cmleiadveabgoenodf)a, maniddeAbHonLdo)x, iadnodreAdHucLtaosxeisd(oorxeidduocretadsuecst(iooxni)d(oFrigeduruect1i)o[n2)].(FAigmuorneg1)th[2e]....