Identification of QuiP, the Product of Gene PA1032, as the Second Acyl-Homoserine Lactone Acylase of Pseudomonas aeruginosa PAO1AnimalsMice, Inbred StrainsMiceLymph NodesSpleenLiverBone Marrow CellsHematopoietic Stem CellsTransplantation, Homologous
Acylases (amido-hydrolases) hydrolyze the amide group and release the homoserine lactone and the carboxylic acid. The currently known acylases separate in two distinct groups: The N-terminal nucleophile aminohydrolases (Ntn hydrolases) and the α/β-hydrolases (Fig.1). Ntn hydrolases cleave an am...
The Pseudomonas aeruginosa PAO1 genome has at least two genes, pvdQ and quiP, encoding acylhomoserine lactone (AHL) acylases. Two additional genes, pa1893 and pa0305, have been predicted to encode penicillin acylase proteins, but have not been characterized. Initial studies on a pa0305 transpos...