and a non-native nucleic acid sequence encoding an alcohol-forming acyl-ACP reductase, in which the recombinant photosynthetic host cell does not express one or more of: a) an exogenous acyl-ACP thioesterase, b) an exogenous acyl-CoA thioesterase and c ) an exogenous acyl-CoA synthetase.HOLTZ...
A gene of a protein having an enzymatic activity enabling the control or regulation of the contents of saturated and unsaturated fatty acids in plant cells and the enzyme protein as the expression product of this gene, more specifically: proteins showing a β-ketoacyl-ACP-synthetase II (KAS II...
Thiolactomycin(16) is another natural product that reversibly inhibitsE. coliFabF, FabB, and FabH with respective IC50’s of 6, 25 and 110µM. Unlike cerulenin, it binds the malonyl-ACP site of the enzyme[27]. Despite modest double-digit MICs onE. coli, S. aureus,Serratia m...
β-Ketoacyl-acyl carrier protein (ACP) synthetase I was purified 180-fold from crude extracts of spinach leaves. The purified preparation was completely free from other component enzymes of the de novo fatty acid synthetase (FAS) system. Its molecular weight was estimated to be 56,000 by gel...
R. Multiple forms of beta-ketoacyl-acyl carrier protein synthetase in Escherichia coli. J. Biol. Chem. 250, 5289–5294 (1975). PubMed Google Scholar Garwin, J., Klages, A. & Cronan, J. Structural, enzymatic, and genetic studies of beta-ketoacyl-acyl carrier protein synthases I and ...
This acyl-CoA pool can be created either by the combination of thioesterase (TE) and acyl-CoA synthetase (e.g., FadD) activity (Option 2) or by acyl-transferase activity (Option 3). Transferase activity (e.g., PhaG) saves one ATP per product relative to Option 2. Detailed pathways ...
6)AcpS and acyl-ACP synthetaseAcpS和脂酰ACP合成酶 延伸阅读 酰基分子式:CAS号:性质:有机或无机含氧酸分子中去掉羟基后,剩下的一价原子团统称为酰基。例如:乙酰基、苯磺酰胺,酰基可与—X、—OR′,—NH2,—HNR′—NR′2′结合生成羧酸衍生物:酰卤,酸酐,酯,酰胺,(N—取代酰胺),(N—二取代酰胺)。羧酸...
Purification and characterization of β-ketoacyl–ACP synthetase I from Spinacea oleracea leaves Arch. Biochem. Biophys. (1983) T.A. McKeon et al. Purification and characterization of the stearoyl–acyl carrier protein desaturase and the Acyl–acyl carrier protein thioesterase from maturing seeds of...
coli ACP with a 4ʹ-phosphopantetheine arm by the acyl-ACP synthetase from Vibrio harveyi38, and then attaching the acyl-chain to the thiol end of the arm using a phosphopantetheinyl transferase (SfP) from Bacillus subtilis. Expression of V. harveyi AasS, B. subtilis SfP and E. coli ...
Pimelic acid, a seven carbon α,ω-dicarboxylic acid (heptanedioic acid), is known to provide seven of the ten biotin carbon atoms including all those of the valeryl side chain. Distinct pimelate synthesis pathways were recently elucidated in Escherichia