and a non-native nucleic acid sequence encoding an alcohol-forming acyl-ACP reductase, in which the recombinant photosynthetic host cell does not express one or more of: a) an exogenous acyl-ACP thioesterase, b) an exogenous acyl-CoA thioesterase and c ) an exogenous acyl-CoA synthetase.HOLTZ...
KasA (beta-ketoacylACPsynthase) coded bykasAgene, is anenzymeinvolved inMAsynthesis. Mdluli et al., reported that in response to INH treatment, a saturated hexacosanoic acid accumulates on ACP, and is referred to as AcpM. However, a second complex with the same amino terminus was fo...
A gene of a protein having an enzymatic activity enabling the control or regulation of the contents of saturated and unsaturated fatty acids in plant cells and the enzyme protein as the expression product of this gene, more specifically: proteins showing a β-ketoacyl-ACP-synthetase II (KAS II...
Purified synthetase I was highly active with acyl-ACP having chain lengths from C 2 to C 14, with hexanoyl-ACP being the most effective substrate, but palmitoyl-ACP was far less effective and stearoyl-ACP almost inactive. The antibiotic, cerulenin, strongly inhibited synthetase I activity. The...
Carbon-carbon bond forming reactions are essential transformations in natural product biosynthesis. During de novo fatty acid and polyketide biosynthesis, β-ketoacyl-acyl carrier protein (ACP) synthases (KS), catalyze this process via a decarboxylative
Anti-ACP antibodies showed no cross-reactivity with the purified acyl-ACP synthetase, indicating no bound ACP with the enzyme. Immunoprecipitations with antibodies to acyl-ACP synthetase revealed that this enzyme is a part of the 10 S triacylglycerol biosynthetic complex. These results demonstrate ...
The Fatty Acid Transport Protein (FATP1) Is a Very Long Chain Acyl-CoA Synthetase The primary sequence of the murine fatty acid transport protein (FATP1) is very similar to the multigene family of very long chain (C20-C26) acyl-CoA synth... NR Coe,AJ Smith,BI Frohnert,... - 《Jour...
We characterized the acyl activating enzyme AAE15 ofArabidopsisencoded byAt4g14070, which is a homologue of a cyanobacterial acyl-ACP synthetase (AAS). We expressed AAE15 in insect cells and demonstrated its AAS activity with medium chain fatty acid (C10–C14) substrates in vitro. Furthermore,...
Acyl-ACP synthetase (AasS) is a member of the adenylate-forming enzymes (Jiang et al., 2006), a ubiquitous enzyme group characterized into three major classes: nonribosomal peptide synthase (NRPS) adenylation domains, acyl- or aryl-CoA synthetases and oxidoreductases in class I; aminoacyl-tRNA...
Two enzymatic activities, 2-acylglycerolphosphoethanolamine (2-acyl-GPE) acyltransferase and acyl-acyl carrier protein (acyl-ACP) synthetase, were solubilized and purified from Escherichia coli membranes. Electrophoretic analysis of the final product of the purification procedure revealed a single protein sp...