(1976). Studies on DNA content, RNA synthesis, and DNA template activity in aging cells of Paramecium aurelia. Expl Cell Res. 98, 63-72... M Klass,J Smithsonneborn - 《Experimental Cell Research》 被引量: 18发表: 1976年 Biochemical studies of the excitable membrane of Paramecium aurelia...
Briefly, following Sanger sequencing of either plasma HIV-1 RNA or cellular HIV-1 DNA,17 maximum-likelihood and Bayesian Markov Chain Monte-Carlo (MCMC) inferences, as implemented in RAxML-HCP2 v8 and MrBayes v3.2.6, respectively, were determined as previously described.18 Controls comprised ...
Mutation of periodic aromatic residues reduced transcriptional activity, whereas increasing the aromatic dispersion of multiple human TFs enhanced transcriptional activity and reprogramming efficiency, promoted liquid–liquid phase separation in vitro and more promiscuous DNA binding in cells. Together with ...
Predicting RNA-seq coverage from DNA sequence as a unifying model of gene regulation Article Open access 08 January 2025 Quantifying the regulatory potential of genetic variants via a hybrid sequence-oriented model with SVEN Article Open access 30 December 2024 Main Deciphering how regulatory funct...
The results also provide the first demonstration of the expression of messenger RNA for 5αR type 1 (5αR1; SRD5A1), 5αR type 2 (5αR2; SRD5A2), 20α-HSO (AKR1C1), 3α-HSO type 2 (3α-HSO2; AKR1C3), 3α-HSO type 3 (3α-HSO3; AKR1C2) and 3β-HSO in breast ...
17, 18, 19, 20, 21, 22 Modified gRNA increased the efficiency of CRISPR/Cas9 and improved the stability of Cas9:RNA:DNA complex as well as reduced off-target effects.23, 24 Cas9 variants enhanced CRISPR/Cas9 activity by increasing the binding threshold to on-target DNA.25, 26 Cas9-based...
Consistent with a role for NHE1 in cell growth regulation, the unbiased microarray analysis indicated that in the absence of NHE1 activity there are significant changes in the expression pattern of genes related to growth factor signaling, growth and oncogenesis, and DNA synthesis and cell cycle ...
TEs are usually separated into two classes that differ in the mode of propagation: retrotransposons, or type I elements, which transpose by reverse transcription of an RNA intermediate; and type II elements, which only use a DNA intermediate in movement within the genome. Both classes can be ...
Recently, cytosine base editors with rAPOBEC1 were reported to induce unguided cytosine deamination in genomic DNA and cellular RNA. Here we report eight next-generation CBEs (BE4 with either RrA3F [wt, F130L], AmAPOBEC1, SsAPOBEC3B [wt, R54Q], or PpAPOBEC1 [wt, H122A, R33A]) ...
DNA and RNA nucleases play a critical role in a growing number of cellular processes ranging from DNA repair to immune surveillance. Nevertheless, many nucleases have unknown or poorly characterized activities. Elucidating nuclease substrate specificitie