scATAC-Seq可在单细胞分辨率下鉴定染色质的开放区域,当在检查异种细胞群体时可以鉴定单个样品中细胞亚群之间染色质可及性的变化。换句话说,普通的ATAC-Seq无法区分样品中亚群的开放染色质区域或亚群之间开放染色质的差异。Active Motif的scATAC-Seq服务中是否包含任何质量控制步骤?是的。一站式的scATAC-Seq服务包含三个...
by Duygu Ucar and Michael Stitzel combines transcriptomic and chromatin accessibility profiling in human pancreatic islet cells to gain new epigenetic insight into the pathogenesis of type 2 diabetes. Their multiomic approach incorporates both ATAC-Seq and RNA-Seq for complete picture of gene expression...
ATAC-seq data from salivary tissue have been published45, but they did not yield additional information. Key TFs controlling mammary function, such as STAT58,9 and ELF57, are dispensable in the salivary gland46. The presence of the progesterone receptor is also required for normal mammary gland...
ATAC-seq Assay for transposase-accessible chromatin with high throughput sequencing (ATAC-seq) is a technique to study chromatin accessibility. It primarily uses DNA transposases following by high-throughput sequencing to provide information on open chromatin regions. Compared to DNase-seq, micrococcal nu...
ATAC-Seq at high sequencing depth across multiple replicates, we have expanded these resources and provided maps of regulatory regions in the trophoblast cell stem state and following differentiation to EVT cells. We analyzed these regulatory regions in multiple ways. First, we called ATAC-Seq peaks...
Zou, Z., Ohta, T., Miura, F. & Oki, S. ChIP-Atlas 2021 update: a data-mining suite for exploring epigenomic landscapes by fully integrating ChIP-seq, ATAC-seq and Bisulfite-seq data.Nucleic Acids Res. ArticleCASPubMedPubMed CentralGoogle Scholar ...
The chromatin immunoprecipitation (ChIP) protocol was performed as previously described,23 with minor modifications. Equal amounts of precleared chromatin were added to antibody-bound Dynabeads, together with Drosophila internal spike-in (Active Motif). ChIP-sequencing (ChIP-seq) experiments were performe...
p, HOMER motif analysis for promoters of genes with the highest ratios of Kacme over H4Kac ChIP-seq signal. Yellow boxes indicate enrichment of motifs associated with E2F family transcription factors. Extended Data Fig. 6 Characterization of H4Kacme antisera. a, Chemical structure of the ...
As expected, differential STARR-seq signal at C1-loci was in line with DNA accessibility, histone modification, and cofactor occupancy dynamics between 2iL and SL (Additional file 1: Fig. S2B). Motif analysis revealed enrichment for TCF/LEF, ZIC2/3, and ZFP281 motifs at 2iL- and SL ...
we scanned the entire genome for motif matches of each of the 14 master TFs, again using HOMER, and selected motif matches with no overlap with the ChIP-seq peaks. IMPACT learns an epigenomic profile representative of cell type regulatory elements in 10-fold cross validation (CV) using the ...