An increase in the expression of genes involved in ubiquitin-mediated proteolysis is observed during many forms of muscle wasting. Hence we analyzed the effect of Myostatin treatment on proteolytic gene expressioCraig McFarlaneAgResearchErin Plummer...
Destruction of Myc by ubiquitin-Mediated proteolysis: cancer-associated and transforming mutations stabilize Myc. EMBO J. 1999; 18:717–726. 21. Wang S, Jiang W, Chen X, Zhang C, Li H, Hou W, Liu Z, McNutt MA, Lu F, Li G. Alpha-fetoprotein acts as a novel signal molecule and ...
Based on the GSEA, PRIM1 might participate in the ubiquitin mediated proteolysis. The protease inhibitor boriezomib reversed the inhibition of P53 induced by PRIM1 overexpression. Thus, we hypothesized that PRIM1 inactivated P53 via ubiquitination-mediated degradation. With the treatment of CHX, PRIM...
It has been shown that TRIM25 uses RNA as a scaffold for efficient ubiquitination of its targets34. We then explored whether ubiquitin-ligase activity of TRIM25 for IGF2BPs is dependent on the presence ofcircNDUFB2. Loss ofcircNDUFB2displayed the notable weaken effects of TRIM25 on the ubi...
GSK-3 beta targets Cdc25A for ubiquitin-mediated proteolysis, and GSK-3 beta inactivation correlates with Cdc25A overproduction in human cancers. Cancer Cell. 2008;13:36–47. Article CAS PubMed PubMed Central Google Scholar Lamorte S, Ferrero S, Aschero S, Monitillo L, Bussolati B, ...
Ubiquitin-mediated proteolysis proceeds via the formation and degradation of ubiquitin-protein conjugates. Ubiquitin (Ub)-activating enzyme (E1) catalyzes the first, MgATP-dependent step in the conjugative reaction sequence. With wild type ubiquitin, the product of the E1 reaction is a ternary complex...
Finally, an important positive regulatory function of DUBs is the recycling of ubiquitin from proteins following their commitment to proteolysis by either the proteasome or lysosome/vacuole [10,11]. There are two known classes of DUBs: the ubiquitin C-terminal hydrolases (UCHs) and the ubiquitin-...
Regulation of NF-kappaB signaling by Pin1-dependent prolyl isomerization and ubiquitin-mediated proteolysis of p65/RelA. Mol Cell 2003; 12: 1413–1426. Article CAS PubMed Google Scholar Ryo A, Nakamura M, Wulf G, Liou YC, Lu KP . Pin1 regulates turnover and subcellular localization of ...
Considering both in vivo and in vitro demonstrations that heated ts85 cells retain the ability to conjugate ubiquitin to endogenous proteins, considerable caution must be exercised in the design and interpretation of proteolysis experiments using this mutant cell line....
G. Short-lived green fluorescent proteins for quantifying ubiquitin/proteasome-dependent proteolysis in living cells. Nat. Biotechnol. 18, 538–543 (2000). Article CAS PubMed Google Scholar Hänzelmann, P. & Schindelin, H. The interplay of cofactor interactions and post-translational modifications...