In dogs a CD4+CD8+ double positive T cell subpopulation exists that has not been phenotypically defined yet. We demonstrate that canine CD4+CD8+ T cells are mature CD1a- and TCR alpha beta + T cells. To analyse
This phenomenon raises an interesting question whether PD-1 harbors positive activity to enhance anti-tumor immunity. Here, we show that PD-1 is secreted in an exosomal form by activated T cells and can remotely interact with either cell surface or exosomal programmed death-ligand 1 (PD-L1),...
Magnetic-activated cell sorting has been applied to the gut microbiome to identify the numbers and types of bacteria eliciting a humoral response. We adapted this technique to examine the populations of immunoglobulin-bound bacteria in the lung. Methods Sixty-four individuals underwent bronchoalveolar ...
on tumor growth. The average body weight ± s.e.m. of the mice bearing tumor xenografts remained generally stable for the duration of the experiment following dosing with the HER2-XPAT proteins or the vehicle control.c, T-cell activation in BT-474 human tumor xenografts and peripheral b...
Figure 2. B cell analysis (A and B) t-Stochastic neighbor embedding (tSNE) plots of B cell from samples with liver/no metastasis (A) and B cell from samples of LCC/RCC with liver/no metastasis (B). (C) tSNE plots showing expression of marker genes for the identified B cell types....
CaV3.1 Does Not Play a Role in T Cell Development and Maturation We then explored a potential role for CaV3.1 in T cell maturation. Analysis of CD4+CD8− single-positive (SP), CD4−CD8+ SP, CD4+CD8+ double-positive (DP), CD4−CD8− double-negative 1 (DN1, CD44+CD25−), ...
PBdMC were positive for tryptase, which is the main mast cell protease released during degranulation [30] (Fig. 1a). Transmission electron microscopy (TEM) showed mature PBdMC containing secretory vesicles (granules) (Fig. 1b), characteristic for mast cells. Although, many of the granules were...
Originally introduced by H. A. Pohl in 1951, dielectrophoretic (DEP) force has been used as a striking tool for biological particle manipulation (or separation) for the last few decades. In particular, dielectrophoresis activated cell sorters (DACSes) ha
In addition, only activated NBs and cells dissociating from them were positive for pERK (Fig. 2e) and c-Jun (Fig. 2g)–markers associated with Schwann cell plasticity and de-differentiation19,20,21. Importantly, cells within activated NBs displayed an increased proliferation rate (quantified as ...
Mallet et al., "Characterization of the MRC OX40 antigen of activated CD4 positive T lymphocytes--a molecule related to nerve growth factor receptor," EMBO J. 9(4):1063-1068 (1990). Mallet et al., "A new superfamily of cell surface proteins related to the nerve growth factor receptor...