Primers for the Target Genes and β-actin Used in RT-PCR.Dehong WuLi XuChengping WenGuanqun XieJinjun JiJieli PanYifeng JiaoYongsheng Fan
The full-length cDNA sequence of Actin gene from the bud of Cymbidium ensifolium was cloned(GenBank accession number :JN613147)by using RT-PCR and RACE with degenerate primer which were synthesized based on the high homologous region among the sequences of several monocotyledon Actin genes...
Primers of phosphate-starvation-inducible (PSI) genes used for RT-PCR analysis Gene OsPHR1 OsPHR2 OsIPS1 OsIPS2 SQD2 OsPAP10 OsActin Primer sequence (5’-3’) F:CACAAGAAGGGAAAACTACCGATG R:TCAAGATTCATGCACTCTACGACGC F:CGCTTTGTAGATGCTGTCAATC R:AGACCCTCATCACATCCTCATTATC F:AAGGGCAGGG...
28S 18S 5S 图 1 海州香薷根部总 RNA 提取 2.2 RT-PCR扩增和克隆 以第一链 cDNA 为模板, 用引物 PrimerF 和 PrimerR 进行 PCR 扩增,获得一条约 550 bp 的条带, 与预期大小相似,将目的条带进行凝胶回收,随后 连接至 pMD18-T 载体上,转化大肠杆菌 TOP10.从 转化平板上随机挑取 5 个白斑进行菌液 PCR...
Cross-intron primers were designed according to the nucleotide sequence of the Actin of Phalaenopsis,and Actin homology fragments from Cymbidium sinense and C.goeringii were obtained by reverse transcription polymerase chain reaction(RT-PCR) and PCR using the first chain of cDNA and the genomic DNA...
Primers of phosphate-starvation-inducible (PSI) genes used for RT-PCR analysis Gene OsPHR1 OsPHR2 OsIPS1 OsIPS2 SQD2 OsPAP10 OsActin Primer sequence (5’-3’) F:CACAAGAAGGGAAAACTACCGATG R:TCAAGATTCATGCACTCTACGACGC F:CGCTTTGTAGATGCTGTCAATC R:AGACCCTCATCACATCCTCATTATC F:AAGGGCAGGG...
多通路 PCR 与单通路 PCR所有包含 VIC™ 报告基因染料标记探针的 TaqMan™ 内源性对照品均为限制性引物。这允许在靶基因表达测定中使用 TaqMan™ 内源性对照品进行多通路测定,前提是对照基因的表达水平远高于靶基因。所有包含 FAM™ 报告基团染料标记探针的 TaqMan™ 内源性对照品均为非限制性引物,并且不...
Here, we show that the SPIRE-type actin nucleators (predominantly SPIRE1) are Rab27a effectors that co-operate with formin-1 to generate actin tracks required for myosin-Va-dependent transport in melanocytes. Thus, in addition to melanophilin/myosin-Va, Rab27a can recruit SPIREs to melano...
In this research, through a method of homology cloning, a pair of degenerate primers were designed based on the conserved sequences of Actin genes from other plants submitted to GenBank, and then 4 cDNA fragments from Cinnamomum camphora were obtained using RT-PCR. Molecular biological analysis ...
The PCR primers used for screening purposes flanked this region (shown in magenta). (C) Sequencing of the PCR-amplified STUB1 locus to which the gRNA targets revealed a single base-pair deletion (highlighted in red) in both alleles. (D) Protein sequence of the mutant CHIP (p.N65TfsX7)...