Thus it appears that the actin-binding site around the two thiols found in skeletal myosin is common to different types of myosin.doi:10.1016/0167-4838(87)90259-7Rie SuzukiFumi MoritaElsevier B.V.Biochim Biophys Acta
The binding of pyrene-actin to myosin was monitored by the decrease in pyrene-fluorescence that accompanies the formation of the actomyosin complex (Fig.5a). The fluorescence transients could be described by single-exponential fits and the respective rate constants were plotted against the actin conc...
The binding of skeletal muscle C-protein to F-actin, and its relation to the interaction of actin with myosin subfragment-1 C-protein is a component of thick filaments of skeletal muscle myofibrils. It is bound to the assembly of myosin tails that forms the filament backbone. We... C Moo...
Constriction of the cytokinetic ring, a circular structure of actin filaments, is an essential step during cell division. Mechanical forces driving the constriction are attributed to myosin motor proteins, which slide actin filaments along each other. Ho
Phosphorylation switches specific for the cardiac isoform of myosin binding protein-C: a modulator of cardiac contraction? Cardiac myosin binding protein-C (cardiac MyBP-C, cardiac C protein) belongs to a family of proteins implicated in both regulatory and structural functions... M,Gautel,O,.....
Myosin binding to actin 喜欢 0 阅读量: 30 作者: Castellani, Loriana,Elliott, Bruce W.,Winkelmann, Donald A.,Vibert, Peter,Cohen, Carolyn 摘要: The actin-binding property of the myosin head 20 K (K = 103 Mr) fragment has been examined by a structural assay. A new fragment is produced...
It has been suggested that the tethering caused by binding of the N-terminal region of smooth muscle caldesmon (CaD) to myosin and its C-terminal region to actin contributes to the inhibition of actin-filament movement over myosin heads in an in vitro motility assay. However, direct evidence ...
The Acanthamoeba myosin-IA heavy chain gene encodes a 134-kDa protein with a catalytic domain, three potential light chain binding sites, and a tail with separately folded tail homology (TH) -1, -2, and -3 domains. TH-1 is highly resistant to trypsin digestion despite consisting of 15% ...
The Structure and Function of the Actin-binding Domain of Myosin Light Chain Kinase of Smooth Muscle In addition to its kinase activity, the myosin light chain kinase (MLCK) of smooth muscle has an actin binding activity through which it can regulate the a... LH Ye,K Hayakawa,H Kishi,.....
Myosin light chain kinase: an actin-binding protein that regulates an ATP-dependent interaction with myosin - ScienceDirect Myosin light chain kinase (MLCK) is a key regulator of smooth muscle contraction. The most conspicuous form of regulation is achieved by phosphorylation of... Kazuhiro,Kohama,...