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Purified PXL1LRR protein (with buffer containing 10 mM Bis-Tris pH 6.0, 100 mM NaCl) was incubated with synthesized peptide mixture (0.1 mg for each; Supplementary Table 1) on ice for 1 h. Then, each mixture was subjected to gel filtration (Superdex 200, GE Healthcare) for ...
Briefly, agarose plugs containing whole-cell DNA of strain WCHEC13-8 were treated with 8 U of S1 nuclease (Fermentas, Thermo Scientific; Waltham, MA, US) and the reaction was stopped by adding 0.5 M EDTA (pH 8). PFGE was conducted with a 1% SeaKem Gold agarose gel (Lonza, Basal, ...
Tab.1 Effects of Jinwu Jiangu decoction containing serum on IL-17 secretion in the supernatant of cultured RA-HFLS x¯±s,n=5 组别剂量/(g·kg-1)IL-17/(ng·L-1) 空白对照组 - 17.50±0.67 雷公藤多苷组 0.36 3.15±0.53*1 金乌健骨方小剂量组 2.4 9.71±0.51*1 金乌健骨方中剂量组 4.8...