We have previously identified a novel type of ACT domain-containing protein family, the ACT domain repeat (ACR) protein family, in itArabidopsis/it. Members of the ACR family, ACR1 to ACR8, contain four copies of the ACT domain that extend throughout the entire polypeptide. Here, we ...
Purified PXL1LRR protein (with buffer containing 10 mM Bis-Tris pH 6.0, 100 mM NaCl) was incubated with synthesized peptide mixture (0.1 mg for each; Supplementary Table 1) on ice for 1 h. Then, each mixture was subjected to gel filtration (Superdex 200, GE Healthcare) for ...
The remaining unique gene was 2,976 bp on a small 3,322 bp contig and contained both an endonuclease/exonuclease/phosphatase and a reverse tran- scriptase domain identified using the InterProScan program. Strain WCHEC13-8 belonged to the phylogenetic group A and a new Sequence type (ST...
Lastly, in silico experiments further revealed that gallic acid could block PKCδ activation by occupying the phorbol ester binding sites of the protein. Keywords: Taiwanese tea; degree of fermentation; catechins; gallic acid; antioxidative activity; RAW264.7; protein kinase C; molecular docking 1. ...