ACK (Ammonium-Chloride-Potassium) Lysing Buffer is used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow. The completeformulationandlysis protocolare available in our Technical Reference Library. ...
ACK (Ammonium-Chloride-Potassium) Lysing Buffer is used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow. The complete formulation and lysis protocol are available in our Technical Reference Library. ...
Pipette 1mL EDTA-treated whole blood into a tube containing 10-20 mL of Gibco™ ACK Lysing Buffer at room temperature. Allow the blood sample plus ACK Lysing Buffer to incubate at room temperature for 3 – 5 minutes. Lysis of the red cells should ...
Animals were genotyped by polymerase chain reaction using murine tissues samples obtained from the ear during the marking of freshly weaned mice according to the genotyping protocol provided by the Jackson Laboratory (JAX, Bar Harbor, Maine, USA) or with the primer listed in the original ...
Cells pellets were lysed in RLB (receptor/cell lysis buffer) (22, 37) on ice for 10 min and sonicated for 25 s to shear DNA to an average length of 300–500 bp. The soluble chromatin was incubated overnight at 4 °C with anti-pTyr-1114 KDM3A, ACK1, and dimethyl H3K9 antibodies ...
P19 cells were subjected to the neural progenitor cell differentiation protocol in the presence or absence of AG1478. Cell lysates were collected at day 9. Black bars and numbers beside the panels indicate the positions and molecular sizes (kDa) of molecular markers. Vinculin (A, D, and G) ...
s protocol (Bio-Rad). Briefly, protein separation was achieved by gel electrophoresis using running buffer (Tris/glycine/SDS from Bio-Rad) for about 1 h with 100–150 Volts (V). Proteins were then transferred from the gel to the membrane in transfer buffer (Tris/glycine buffer from Bio-...
cells were starved for 4 h at 37 °C in serum-free medium. Cells were then stimulated for the indicated time intervals with chemokine or opioid ligands. The supernatants were replaced with the lysis buffer provided and incubated 1.5 h. Lysates were transferred to white 384-well plates ...
Results Single-cell transcriptome atlas of human tendon adhesion tissue To determine the cellular composition of tendon adhesion tissue, tissue samples were obtained surgically from 12 patients with flexor tendon tenolysis including three samples of normal peritendinous tissue and three pathologic samples ...