generates malonyl-CoA under the catalysis of ACC, thereby participating in the de novo synthesis of fatty acids, and on the other hand, it can enter the nucleus for histone acetylation modification. Acetate and citrate can enter the nucleus as raw materials for the production of acetyl-CoA. AC...
DNL is carried out in the cytosol by three enzymes, ATP citrate lyase, acetyl-CoA carboxylase (ACC), and fatty acid synthase (FASN). ATP citrate lyase produces acetyl-CoA, the substrate for acetyl-CoA carboxylase (ACC), which produces malonyl-CoA. Fatty acid synthase, a multifunctional enzyme...
Cholesterol is synthesized in chicken through de novo lipid biosynthetic pathway where two most important genes viz. SREBP1 and ACACA play immense role. To minimize cholesterol synthesis, RNAi approach was adopted and accordingly, we developed transgenic
Both ACC1 and ACC2 convert acetyl-CoA to malonyl-CoA, a substrate for DNL and an allosteric inhibitor of CPT1. This enzyme catalyzes the rate-limiting step of FAO. In tumor cells, abnormal regulation of ACC by AMPK, mTOR, and BRCA1 promotes tumorigenesis via converting acetyl-CoA to ...
CoA because ACC1 is an acetyl-CoA binding protein, which facilitates the enrichment of other CoA binding proteins, such as FASN; thus, SA expression could simultaneously sequester ACC1 and FASN, which helps to explain why SA expression led to both increased acetyl-CoA and malonyl-CoA contents....
Detection of ACC activity and malonyl-CoA levels Liver homogenates or cell lysates were assayed for ACC enzymatic activity using enzyme-linked immunosorbent assay (ELISA) (Solarbio Life Science, Beijing, China) and malonyl-CoA levels using enzyme-linked immunosorbent assay (Cusabio, Houston, TX, USA...
Acp1HA/FLAG and the LYRV5 fusion proteins were expressed in strains in which MCT1 or OAR1 had been deleted (mtFASΔ). These genes encode the malonyl-CoA:ACP transferase (MCT1) and the 3-oxoacyl-ACP reductase (OAR1) and are both required for mtFAS activity (Figure S2A) (Hiltunen et...