Increasing the amount of DNA polymerase from 1 to S U had a strong effect for ExTaq HS, elevating HA-tolerance four times. We also show that the average Cq values of positive reactions may be used as a measure of inhibition effects, e.g., to determine whether or not a dPCR ...
FastTaq Premix(with dye) 10 *1 mL 50 mL 1,000 mL FastPfu DNA polymerase 100 U 500 U 1,000 U FastPfu Premix 1 mL 10*1 mL 50 mL 1,000 mL FastPfu Premix (with dye) 1 mL 10*1 mL 50 mL 1,000 mL P6 High-Fidelity polymerase 100 U 500 U 1,000 U P6 High-Fidelity Premix 1...
was ple was heated at picked from agar plates and re-suspended in 30 μ l 96 °C for 5–10 minutes to lyse the cells, and 1 μ l of otfhidsHs2uOspienns0i.o2n mwl atus buesse.dTahse sam- DNA template in 30 μ l PCR reactions using Taq DNA polymerase. ...
The on-site production of functional amplicons was performed on a portable thermal controller with the volume of 25 µL containing: 12.5 µL 2 × PCR Mix buffer (2.0 mM MgCl2, 200 µM of dNTPs and 5 U Taq DNA polymerase); 1 µL functional forward primer (10 μM) and reverse ...
Second-strand cDNA synthesis was subsequently performed using buffer, dNTPs, DNA polymerase I, and RNase H. The library fragments were purified with QiaQuick PCR kits and eluted with EB buffer, then terminal repair, A-tailing, and adapter addition were implemented. The target products were ...
To this aim, long-range PCR was performed using the High-Fidelity LA Taq DNA Polymerase (Takara) according to the manufacturer's recommendations and the oligonucleotide primers RAX2_del1_F: 5 -TGTTACCCACACCATTCTCTGC-3 and RAX2_del1_R: 5 -CCCTCTCCTTTCCATCTCTAG-3 . Amplicons spanning ...
PCR amplification was performed using a Bio-Rad C1000 Touch Thermal Cycler (Bio-Rad Laboratories) in 10 µL reaction mixtures containing 20 ng of genomic DNA, 0.4 µM of each primer and 1× MyTaq Mix following the recommendations of the manufacture (Bioline). The following PCR cycling ...