To simultaneously improve product yield and amplification fidelity,Tba5 plus DNA polymerase mixtures were constituted with various amounts ofTba5 DNA polymerase mixed withTaqDNA polymerase. TheTba5 plus DNA polymerase mixtures robustly amplified up to 25-kb λ DNA fragments. In addition, the PCR ...
The spike-in control, including 3 distinct lambda DNA amplification products (~180 bp; one without modification and the other two with 5mC and 5hmC modifications), was prepared as follow47: lambda DNA was PCR amplified by Taq DNA Polymerase (NEB) and purified by AMPure XP beads (Beckman...
Total RNA isolation and complementary DNA synthesis Total RNA was extracted using the Nucleospin® RNA Plant kit (Macherey–Nagel, Düren, Germany), starting from 100 mg of finely grinded leaf samples, according to the manufacturer’s instructions. RNA concentration was estimated by reading spectrop...
-α4.2, –SEA, –FIL, –THAIwere characterised using an adapted multiplex-PCR21(Supplementary Table 1). Hot StartTaqDNA polymerase (Qiagen HotStarTaqPlusDNA Polymerase, Hilden, Germany) was used for DNA amplification. The modified PCR conditions for DNA amplification were as follows: initial denatu...
(NH4)2SO4, 3 mmol l − 1 MgCl2, 50 μmol l − 1 each dNTP, 480 nmol l − 1 of each specific primer for SEA deletion, 900 nmol l − 1 of each primer for SNP (rs3760053), 1 μmol l − 1 SYBR green and 0.02 units Taq DNA polymerase (Biolabs Co., Ltd., Ipswich,...
For each sample, two duplex PCRs were carried out using 5 ng genomic DNA and 0.5 U Taq DNA polymerase (NEB) in a buffer with final concentrations of 50 mM Tris–HCl pH8.8, 12.5 mM ammonium sulphate, 1.4 mM magnesium chloride, 7.5 mM 2-mercaptoethanol, 125 μg/ml BSA and 200 μM ...
FastTaq Premix(with dye) 10 *1 mL 50 mL 1,000 mL FastPfu DNA polymerase 100 U 500 U 1,000 U FastPfu Premix 1 mL 10*1 mL 50 mL 1,000 mL FastPfu Premix (with dye) 1 mL 10*1 mL 50 mL 1,000 mL P6 High-Fidelity polymerase 100 U 500 U 1,000 U P6 High-Fidelity Premix 1...
For the pre- and post-capture PCR reaction, we tested the performance of Tks Gflex™ DNA Polymerase (Takara Bio Inc., Japan) and LongAmp™ Taq 2× Master Mix (New England Biolabs, Ipswich, MA, USA) for 1000 bp fragment amplification. The use of LongAmp™ Taq with PRM primers incl...
PCR with variant-specific primers (Integrated DNA Technologies) using the Taq DNA-Polymerase (Thermo Fisher Scientific, Waltham, MA, USA) or the OneTaq DNA Polymerase (New England Biolabs) with GC-Rich Buffer was performed for breakpoint confirmation. Primer sequences are available upon request....
Nuclear mitochondrial DNA ONT: Oxford Nanopore Technologies PCR: Polymerase chain reaction PERMANOVA: Permutational multivariate analysis of variance SFB: Short fragment buffer SUP: Super accurate WoRMS: World Register of Marine Species del Socorro Toxqui Rodríguez M, Naya-Català F, Sitjà-Bobadilla A...