While its use in these fielde has already contributed to great advancements and revolutionary changes, current PCR using Taq DNA polymerase is usually limited to amplification up to 5kb. Recently, "long" PCR conditions have been identified which allow the amplification of DNA templates up to 40kb...
Increasing the amount of DNA polymerase from 1 to S U had a strong effect for ExTaq HS, elevating HA-tolerance four times. We also show that the average Cq values of positive reactions may be used as a measure of inhibition effects, e.g., to determine whether or not a dPCR ...
was prepared as follow47: lambda DNA was PCR amplified by Taq DNA Polymerase (NEB) and purified by AMPure XP beads (Beckman Counlter) in nonoverlapping ~180 bp amplicons, with a cocktail of dATP/dGTP/dTTP and one of the following
FastTaq Premix(with dye) 10 *1 mL 50 mL 1,000 mL FastPfu DNA polymerase 100 U 500 U 1,000 U FastPfu Premix 1 mL 10*1 mL 50 mL 1,000 mL FastPfu Premix (with dye) 1 mL 10*1 mL 50 mL 1,000 mL P6 High-Fidelity polymerase 100 U 500 U 1,000 U P6 High-Fidelity Premix 1...
FastTaq Premix(with dye) 10 *1 mL 50 mL 1,000 mL FastPfu DNA polymerase 100 U 500 U 1,000 U FastPfu Premix 1 mL 10*1 mL 50 mL 1,000 mL FastPfu Premix (with dye) 1 mL 10*1 mL 50 mL 1,000 mL P6 High-Fidelity polymerase 100 U 500 U 1,000 U P6 High-Fidelity Premix 1...
DNA analysis for deletional α-thalassaemia The five common deletions causing α-thalassaemia in Southeast Asia: -α3.7, -α4.2, –SEA, –FIL, –THAIwere characterised using an adapted multiplex-PCR21(Supplementary Table 1). Hot StartTaqDNA polymerase (Qiagen HotStarTaqPlusDNA Polymerase, Hilden,...
Quantitative reverse transcription-polymerase chain reaction to study mRNA decay: comparison of endpoint and real-time methods. Anal Biochem. 2000;285(2):194–204. Article CAS Google Scholar Tichopad A, Dilger M, Schwarz G, Pfaffl MW. Standardised determination of real-time PCR efficiency from...
aBased on the instruction of manufacturer, the PCR reaction (25 μL) used 0.125 μL of Taq polymerase (1.25 U), 0.5 μL of primers (5 pmol), 1 μL of 10-fold diluted DNA template (approximately 1 ng), 2.5 μL of 10-fold PCR buffer, 1.5 μL of MgCl2 (50 mM ), and lastly...
(NH4)2SO4, 3 mmol l − 1 MgCl2, 50 μmol l − 1 each dNTP, 480 nmol l − 1 of each specific primer for SEA deletion, 900 nmol l − 1 of each primer for SNP (rs3760053), 1 μmol l − 1 SYBR green and 0.02 units Taq DNA polymerase (Biolabs Co., Ltd., Ipswich,...
FastPfu DNA polymerase 21803-01 100 U 21803-02 500 U 21803-03 1,000 U P6 High-Fidelity polymerase 21804-01 100 U 21804-02 500 U 21804-03 1,000 U 2× P6 High-Fidelity Premix 21805-01 1 mL 21805-02 10 *1 mL 21805-03 50 *1 mL 反转录酶 ToloScript Reverse Transcriptase 22101-01...