在这五年的 western blot 实验历程里,我先后用过进口抗体,进口抗 体国内分装包装,国产抗体,质量良莠不齐。 进口抗体一般不会出现闪失:abcam 品种全,质量过硬,但价高(3400元/100微升) ,而 且说是100微升,但至多能吸出来90微升;Sigma 的价最贵;比较有性价比的是 CST 的抗 体,现在好像是2200元/100微升,我...
在这五年的western blot实验历程里,我先后用过进口抗体,进口抗体国内分装包装,国产抗体,质量良莠不齐。 进口抗体一般不会出现闪失:abcam品种全,质量过硬,但价高(3400元/100微升),而且说是100微升,但至多能吸出来90微升;Sigma的价最贵;比较有性价比的是CST的抗体,现在好像是2200元/100微升,我前后用过二十几种, ...
五年的westernblot经验总结(附abcam的wb的protocol) 交流 原创与否- 本帖最后由mche2005于11-1-210:59编辑 1.抗体的选择 对于国内的大多数实验室来讲,做westernblot实验选择抗体是个头疼的问题。原因很简 单,买进口抗体捉襟见肘,买国产抗体得需要大无畏的勇气,对于我所在的兰州地区的实验 ...
Dot blot is a technique for detecting, analyzing, and identifying proteins. This technique is similar towestern blot, but protein samples are not separated usingelectrophoresis; instead, proteins are spotted through circular templates directly onto the membrane or paper substrate. The concentration...
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I\'m sorry to hear that there were no bands in Western blot using this antibody. I would expect any of the MEF cell lines to work as a positive control, but I suggest using a whole cell lysate (ab14874 is a nuclear fraction) since that is what we have tested with ab80. What kind...
Western blot - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791) All lanes :Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (ab1791) at 1 µg/mlLane 1 :HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 :NIH 3T3 whole cell lysate (...
内容提示: www.abcam.com/technical IMMUNOPRECIPITATION (IP) PROTOCOL Immunoprecipitation is a method that enables the purification of a protein. An antibody for the protein of interest is incubated with a cell extract so that the antibody will bind the protein in solution. The antibody/antigen ...
catalog : ab6311 quantity : 500 µl clonality : monoclonal host : mouse conjugate : nonconjugated clone name : COL-94 reactivity : human,rat application : western blot,ELISA,immunohistochemistry,immunocytochemistry,immunohistochemistry - paraffin section,immunohistochemistry - frozen section,dot blot ...
Key protocols including: · Western blot · ChIP · ELISA · Flow cytometry · Immunocytochemistry · Immunohistochemistry · ELISA Bench tools · Protocol timers · Molarity calculator · Dilution calculator – mass · Calculator – molar · Unit converter ...