This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin or 3% Milk befo...
Dot blot is a technique for detecting, analyzing, and identifying proteins. This technique is similar towestern blot, but protein samples are not separated usingelectrophoresis; instead, proteins are spotted through circular templates directly onto the membrane or paper substrate. The concentration...
裂解物说明 Mouse NIH/3T3 cell lysate was prepared by homogenization in modified RIPA buffer(50 mM Tris-HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate,1 mM phenylmethyl-sulfonyl flouride, 5 µg/ml of aproti...
short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.Storage buffer pH: 7.00 Preservative: 0.025% Proclin 300 Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)Purity Immunogen affinity purified Clonality Polyclonal Isotype IgG 1 ...
StoragebufferConstituents:0.09%Sodiumchloride,0.02%Dibasicmonohydrogensodiumphosphate,50%Glycerol PurityImmunogenaffinitypurified ClonalityPolyclonal IsotypeIgG CellularlocalizationSecretedprotein;extracellularspace. Overview Properties Applications OurAbpromiseguaranteecoverstheuseofab103789inthefollowingtestedapplications. ...
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This blot was produced using a 4-12% Bis- Tris gel under the MOPS buffer system. The gel was run at 200V for 60 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour ...
Western Blot Stripping Buffer ab171529 450 nm Stop Solution for TMB Substrate ab235138 Rhodamine Phalloidin Reagent ab286858 1X Red Blood Cell Lysis Buffer ab286850 Density Gradient Medium (Iodixanol) ab267832 GAPDH positive control ChIP primer pair ab288118 Lyophilized Exosome Standard (Human Plasma)...
· Western blot · ChIP · ELISA · Flow cytometry · Immunocytochemistry · Immunohistochemistry · ELISA Bench tools · Protocol timers · Molarity calculator · Dilution calculator – mass · Calculator – molar · Unit converter · Buffer receipes · Fluorochrome guide · Abcam application abbrevia...
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64693, 0.1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system...