AAV9-retroAcross the blood–brain barrierAdeno-associated virusesNeural circuitsRetrogradelyRecombinant adeno-associated viruses (rAAVs), particularly those that permit efficient gene transfer to neurons from axonal terminals or across the blood鈥揵rain barrier, are useful vehicles for structural and ...
Second, post-mortem analysis revealed expression of GFP in thalamic and cortical neurons as well as dopaminergic neurons projecting from substantia nigra pars compacta, indicating retrograde transport of AAV9. However, fibers in the substantia nigra pars reticulata, a region that receives projections ...
Kaspar, B.K. et al. Targeted retrograde gene delivery for neuronal protection.Mol. Ther.5, 50–56 (2002). ArticleCASGoogle Scholar Miller, T.M. et al. Gene transfer demonstrates that muscle is not a primary target for non-cell-autonomous toxicity in familial amyotrophic lateral sclerosis.Pro...
After 2 weeks, when the pigs displayed significant left ventricular contractile dysfunction, we administered, by retrograde coronary venous delivery, AAV serotype 9 (AAV9)-S100A1 to the left ventricular, non-infarcted myocardium. AAV9-luciferase and saline treatment served as control. At 14 weeks,...
Sauer H, Oertel WH . Progressive degeneration of nigrostriatal dopamine neurons following intrastriatal terminal lesions with 6-hydroxydopamine: a combined retrograde tracing and immunocytochemical study in the rat.Neuroscience1994;59: 401–415.
We hypothesized that the administration of AAV9 vector by retrograde infusion via the ureter can provide efficient gene transfer to renal cells and minimize off-target gene expression. AAV9 vector (1e+11 viral genome particles/100 渭l), encoding firefly luciferase (AAVCMVLuc) or eGFP (AAV...
Gene targeting of GAN in mouse causes a toxic accumulation of microtubule-associated protein 8 and impaired retrograde axonal transport Hum. Mol. Genet., 15 (2006), pp. 1451-1463 CrossrefView in ScopusGoogle Scholar 34 F. Dequen, P. Bomont, G. Gowing, D.W. Cleveland, J.P. Julien Mode...
In fact, AAV9 can travel along neuronal axons in both retrograde and anterograde manners,49,50 and we cannot exclude AAV muscle targeting through nerve retrograde transport. The kinetic serum shedding analysis revealed the presence of viral particles in serum at 1 h (which represented the higher ...
After entry, AAV was trafficked into nonmotile early and recycling endosomes, exocytic vesicles, and a retrograde-directed late endosome/lysosome compartment. Rab7-positive late endosomes/lysosomes that contained AAV were highly motile, exhibiting faster retrograde velocities and less pausing than Rab7-...
strated that AAV8 is effective in retrograde transport from muscle to spinal cord and DRG [13]. Foust et al. reported that a single intravenous injection of self- complementary AAV9 into newborn mice provided gene transfer across the blood–brain barrier (BBB) and into ...