AAV9-Retro不仅具有与AAV2-Retro相当的逆行转导效率，还保留了AAV9血清型跨血脑屏障的特性。成年小鼠尾静脉注射AAV9-Retro-EGFP后，在皮层、纹状体、苍白、海马体、丘脑、下丘脑等重要的脑区均有荧光信号，且其转导效率与AAV9血清型相当。因此，AAV9-Retro用于外周递送基因进行上行标记具有更大的潜力。AAV9-Retro...
AAV9-Retro不仅具有与AAV2-Retro相当的逆行转导效率,还保留了AAV9血清型跨血脑屏障的特性。成年小鼠尾静脉注射AAV9-Retro-EGFP后,在皮层、纹状体、苍白、海马体、丘脑、下丘脑等重要的脑区均有荧光信号,且其转导效率与AAV9血清型相当。因此,AAV9-Retro用于外周递送基因进行上行标记具有更大的潜力。 AAV9-Retro血...
AAV9-retroAcross the blood–brain barrierAdeno-associated virusesNeural circuitsRetrogradelyRecombinant adeno-associated viruses (rAAVs), particularly those that permit efficient gene transfer to neurons from axonal terminals or across the blood鈥揵rain barrier, are useful vehicles for structural and ...
AAV5和AAV9衣壳的开发 目前报道的适用于神经系统的AAV血清型有:1型、2型、5型、6型、8型、9型、DJ、PHP.B、PHP.eB、PHP.S、Retro、rh10等十几种。但对于中枢神经系统的治疗,AAV5型和AAV9型相对具有更好的扩散能力。 相对于其他分型,AAV5和AAV9在一些中枢神经区域的扩散能力和转染效率均处于前列。但是免...
A new AAV variant, AAV9-Retro, was developed by inserting the 10-mer peptide fragment from AAV2-Retro into the capsid of AAV9, and the biodistribution properties were evaluated in mice. By intracranial and intravenous injection in the mice, we found that AAV9-Retro can retrogradely infect ...
Full size image Figure 2 Molecular characterization of AAV2/9-CMV-hNDUFS4 IV-treated adult mice. A single retroorbital injection of 2 × 1012vg was performed inNdufs4−/−mice at P21. (a) Western blot analysis of tissue homogenates. (b) Comparison ofNdufs4+/+liver,Ndufs4−/−...
(Jackson Labs #026267) were retro-orbitally administered 1 × 1012 viral genomes (v.g.) per animal AAV1-X1 packaging either EF1a-mCherry or EF1a-Cre (N = 6 per condition). After 3 weeks, mice were re-administered 1 × 1012v.g. per animal PHP.eB or AAV-X1.1 ...
A single retroorbital injection of 2 × 1012 vg was performed in Ndufs4−/− mice at P21. (a) Western blot analysis of tissue homogenates. (b) Comparison of Ndufs4+/+ liver, Ndufs4−/− AAV-treated liver and human fibroblasts (hFib) homogenates. SDH- A, the 70 kDa subunit...
进一步与AAV2-Retro进行比较,结果发现这两种血清型可以感染相同的脑区(VTA、CPU)以及其上游脑区,且两者逆行感染效率相当(图1、图2)。文章中运用具有逆行标记效率的AAV9-Retro血清型为病毒…
4 delivered their injections into the retro-orbital sinus, the venous sinus located behind the eye. The proximity of this sinus to the brain could mean more of the vector is immediately accessible to the brain, before being circulated and cleared from the bloodstream, as compared with injection...