AAV2 VP 蛋白标准品可用于创建具有精确摩尔比(例如 1:1:10)的VP1、VP2 和 VP3 混合物,以便使用蛋白质印迹等蛋白质检测方法与您的样品的蛋白质组成进行比较。所有三种 AAV2 蛋白均可作为单独的蛋白质(每种10 µg)提供,并可根据需要用于制备特定的 VP 蛋白混合物。
Mutagenesis of Aav2 Capsid Protein Vp1 Uncovers New Roles for Basic Amino Acids in Trafficking and Cell-Specific TransductionJohnson, J SLi, CDiprimio, NWeinberg, M SMcCown, T JSamulski, R J
changes in the protein sequences of the capsid proteins might also influence conformation of the proteins, hence the conformation of the epitopes presented on the AAV capsid. This might influence binding affinity of the antibody and affect determination of the titer based on the (non-shuffled) Kit...
pRC'99 was generated by inserting the overlapping sequence of the restriction enzymes MluI, BspEI, and AscI into the amino acid position I-587 of the AAV2 capsid cloned in pRCwt. The pXX6 adenovirus helper plasmid [48] was provided by Richard J. Samulski. Packaging of AAV with wild-type...
Only VP1, VP2, and VP3 proteins in the typical stoichiometry of approximately 1:1:10 were detectable, indicating a purity of the AAV preparation of >95%. Finally, AAV samples were adjusted to a titer of 1013 vg/ml, aliquoted, and frozen at −80 °C. In vitro differentiation, ...
1The amino acid positions in the AAV2 capsid VP1 protein are 455-461, 663-667 and 713-717 for Epitopes 1, 2 and 3 respectively. All references cited in this disclosure are incorporated by reference in their entirety. It will be apparent to those having skill in the art that many changes...
per- formed an in vivo with a mixture of different AAV capsid libraries, and identified an enriched capsid clone from a 7-mer peptide AAV2 capsid library (peptides inserted after amino acid 588 in VP1). This capsid clone enhanced SCIENTIfIC Reports | 7:45329 | DOI: 10.1038/srep45329 6 ...
Fig. 1. Multiple sequence alignment of 140 AAV VP1 sequences. (a) A sequence logo illustrates the conservation of D154, S155, S156, S157, and G158 residues in AAV. The bit height profile measures the certainty and frequency levels for conservation of each residue. (b) Prevalence of D...
“Adeno-associated virus 5 DNA binding trs helicase (Rep22) and capsid protein (VP1) genes,complete cds,” 2 total pages. GenBank Accession No. NC_001862.1 (2004). “Adeno-associated virus-6, complete genome,” 3 total pages. GenBank Accession No. AX753246.1 (2003). “Sequence 1 ...
terminal repeat (ITR)AAV2 capsid proteinThe AAV2 capsid protein containing an amino acid sequence lgettrp (array number 14) inserted between 587 and 588 of the capsid proteinThe numbering of the amino acid residues corresponds to the AAV2 VP1 capsid protein.The above method is provided.No ...