This dissertation includes a series of studies investigating the effects of nigrostriatal PTN overexpression by injection of recombinant adeno-associated viral vectors (rAAV) in both the 6-hydroxydopamine (6-OHDA) and human wild-type alpha-synuclein (α-syn) overexpression (AAV) rat models of PD....
diseasemodels.NatureNeuroscience,2006,9(2):251～259 Overexpressionofα?SynucleinGeneCausedDopaminergicNeuron DamageinSubstantiaNigraofRats LULing?ling ZHOUAi?xia YangHui (BeijingInstituteforNeuroscience,CapitalMedicalUniversity,BeijingCenterofNeuralRegenerationandRepair,KeyLaboratoryfor NeurodegenerativeDiseaseoftheMinis...
However, given their lack of α-synuclein aggregation and Lewy body formation, these models poorly reflect the molecular aspects of the human disease. The role of the adaptive immune system thus needs to be assessed in pathogenetically more relevant model systems. Williams et al. recently tested ...
Studies using in vivo models to evaluate the efficiency, specificity, and safety of AAV-delivered CRISPR/dCas9 platforms serve as the “gold standard” in the preclinical stages of drug development. To the best of our knowledge, no study thus far has reported successful epigenetic editing in ani...
Mouse γ-synuclein promoter-mediated gene expression and editing in mammalian retinal ganglion cells. J Neurosci. 2020;40:3896–914. Article CAS PubMed PubMed Central Google Scholar Boyd RF, Sledge DG, Boye SL, Boye SE, Hauswirth WW, Komáromy AM, et al. Photoreceptor-targeted gene delivery...
This model further provides insights into potential therapeutic approaches, particularly concerning α-synuclein accumulation and its subsequent pathological consequences. 展开 关键词: RAPID eye movement sleep SLEEP interruptions SLEEP PARKINSON'S disease EYE movements ...
All mouse strains below were confirmed to be Crb1rd8-negative, Nnt(exon 7–11)WT/WT, Mmrn1(exon 8)−/−,α-synuclein(exon 6)−/−, and Pde6bWT/WT, thus similar to the genetic background of C57BL/6JOlaHsd mice. Crb1KOCrb2Flox/Flox mice were crossed with a Crb1KOPdgfrα...
Results in animal models of other age-related disorders suggest NNI-362 has the potential to halt or reverse AD and PD clinically. Method An AAV-alpha synuclein rat model of Parkinson's disease (PD) (MOTAC, France) was used to examine the effects of NNI-362 treatment. This model results ...
The accumulation of misfolded α-synuclein in dopamine (DA) neurons is believed to be of major importance in the pathogenesis of Parkinson's disease (PD). Animal models of PD, based on viral-vector-mediated over-expression of α-synuclein, have been developed and show evidence of dopaminergic ...
Adeno Associated Virus (AAV)-mediated gene expression in the brain is widely applied in the preclinical setting to investigate the therapeutic potential of specific molecular targets, characterize various cellular functions, and model central nervous system (CNS) diseases. In therapeutic applications in ...