The AAV.S2 capsid provided 2.31 卤 0.37-fold higher expression of miRNA over that provided by AAVrh.10 (p < 0.05). In the targeted region, a single intra-hippocampus AAV.S2 administration suppressed hippocampal APOE4 mRNA levels by 76.5 卤 3.9% compared with 41.3 卤 3.3% with the same ...
Use this AAV Rep-Cap plasmid along with the AAV Helper Free System to produce recombinant human adeno associated virus (rAAV) virions without the need for a helper virus Ideal if you already have the AAV Helper Free System with a different AAV serotype Frequently Asked Questions about AAV ...
For our recombinant AAV manufacturing, the transfer plasmid carrying the gene of interest (GOI) is co-transfected with our proprietary Rep-cap plasmid and helper plasmid encoding adenovirus genes (E4, E2A and VA) that mediate AAV replication into HEK293T packaging cells. After a short incubation...
The platform process was developed for standard AAV serotypes AAV2, 5, and 6 in-house and further evaluated for customer projects for several others including 1, 2, 5, 6, 8, 9, and rh10. Starting with Catalent off-the-shelf GMP plasmids and producer cells, the UpTempo℠ platform can...
For instance, if it is rAAV2/5 that is to be produced based on the helper-free system discussed above, the AAV packaging plasmid AAV-RC should be pRep2/Cap5, that is, the Rep gene is derived from AAV2, while the Capsid gene from AAV5. The packaging scheme is the same as the ...
When the cells reached 80% confluence, they were transfected in 150 mm plates with 12 μg of pHelper plasmid, 10 μg of AAV pRep2CapX plasmids, and 6 μg of transfer AAV plasmids encoding the cargo for each plate. At 72 h post transfection, cells were harvested by 4000...
Helper genes: Plasmid Cell line (Species): HEK293 (H. sapiens) HSV Infection GOI: rHSV #1 ∗Major consideration: supply for HSV for larger AAV production volumes ∗ Linear, relatively direct scalability of rAAV production [50] rep/cap AAV genes: rHSV #2 Helper genes: Provided by rHSV ...
(RFU) are plottedversustemperature, and the melting point is determined as the temperature that corresponds to the apex at a maximum RFU (Fig.6). The study by Bennettet al. (124) on AAV1–9 and AAVrh.10 showed that those AAV serotypes exhibited distinct, specific melting temperatures ...
The variant AAV capsid can have an AAV serotype of AAV9, AAV9 K449R (or K449R AAV9), AAV1, AAVrh10, AAV-DJ, AAV-DJ8, AAV5, AAVPHP.B (PHP.B), AAVPHP.A (PHP.A), AAVG2B-26, AAVG2B-13, AAVTH1.1-32, AAVTH1.1-35, AAVPHP.B2 (PHP.B2), AAVPHP.B 3 (PHP.B3), ...
A modified AAV2/9 Rep-Cap plasmid may be provided, wherein stop-codons are provided in-frame to prevent the expression of VP1-VP3 proteins. Capsid libraries are generated using the rAAV-Cap-in-cis-lox viral genome as a backbone. Targeting peptides are inserted into the parent AAV capsid...