顺向(Anterograde)是由注射部位的神经元胞体经轴突下行至末梢，逆向(Retrograde)则是由轴突末梢传到细胞体；顺向跨突触是从胞体传到轴突末梢后跨过突触传递至下一级神经元，逆向跨突触跟顺向跨突触相反，是由胞体逆向跨突触传递至上一级神经元的轴突。如下图所示：（Strick P L，et al.，eLS，2011）腺相关病毒（Ade...
利用神经示踪工具实现对神经环路的精确标记,主要涉及两大基本问题即方向性(顺向、逆向)和是否跨突触。顺向(Anterograde)是由注射部位的神经元胞体经轴突下行至末梢,逆向(Retrograde)则是由轴突末梢传到细胞体;顺向跨突触是从胞体传到轴突末梢后跨过突触传递至下一级神经元,逆向跨突触跟顺向跨突触相反,是由胞体逆向跨突...
神经研究中『可逆向示踪』的AAV血清型-AAV2-retro 神经研究中实现对神经环路的精确标记,常需考虑示踪方向性即顺逆向问题。顺向(Anterograde)是由注射部位的神经元胞体经轴突下行至末梢,逆向(Retrograde)则是由轴突末梢传到细胞体。 图1. 神经元的顺向及逆向标记(Strick P L et al. eLS, 2011) 传统的逆向标记神...
[2].Chung, D.C., et al., Adeno-Associated Virus-Mediated Gene Transfer to Renal Tubule Cells via a Retrograde Ureteral Approach. Nephron Extra, 2011. 1(1): p. 217-223.[3].Rocca, C.J., et al., rAAV9 combined with renal vein injection is optimal for kidney-targeted gene delivery:...
顺向(Anterograde)是由注射部位的神经元胞体经轴突下行至末梢,逆向(Retrograde)是由轴突末梢传到细胞体;顺向跨突触(Anterograde transneuronal)是从胞体传到轴突末梢后跨过突触传递至下一级神经元,逆向跨突触(Retrograde transneuronal)跟顺向跨突触相反,是由胞体逆向跨突触传递至...
[2]. Chung, D.C., et al., Adeno-Associated Virus-Mediated Gene Transfer to Renal Tubule Cells via a Retrograde Ureteral Approach. Nephron Extra, 2011. 1(1): p. 217-223. [3]. Rocca, C.J., et al., rAAV9 combined with renal vein injection is optimal for kidney-targeted gene deli...
[2]. Chung, D.C., et al., Adeno-Associated Virus-Mediated Gene Transfer to Renal Tubule Cells via a Retrograde Ureteral Approach. Nephron Extra, 2011. 1(1): p. 217-223. [3]. Rocca, C.J., et al., rAAV9 combined with renal vein injection is optimal for kidney-targeted gene deli...
中科院徐富强团队于2022年初发表在《分子脑（Molecular Brain）》上题为“Brain-wide TVA compensation allows rabies virus to retrograde target cell-type-specific projection neurons”的文章即据此设计了“AAV-PHP.eB辅助的逆行标记系统”（AAV-PHP.eB-Assisted Retrograde Tracing Systems，即PARTS系统），以及“细胞...
神经研究中实现对神经环路的精确标记,常需考虑示踪方向性即顺逆向问题。顺向(Anterograde)是由注射部位的神经元胞体经轴突下行至末梢,逆向(Retrograde)则是由轴突末梢传到细胞体。 传统的逆向标记神经元的工具包括荧光染料等示踪剂,这类工具促进了人们对大脑神经网络结构的认识,但也存在以下缺点:①无法特异性标记细胞类型...
These results may suggest a lack of DRN axon terminals within the viral injection sites or a failure of AAV retrograde tracer transduction in DRN projections to the BLA. Future studies comparing traditional retrograde tracer techniques to our results are warranted. In sum, these results caution the...