注射参数病毒类型: AAV-hSyn-HA-hM4Di-IRES-mCitrineAAV-CaMKIIα-eGFPAAV-hsyn-DIO-hM3Dq-eGFPAAV-hsyn-DIO-hM4Di-eGFPAAV-hsyn-DIO-mCherryAAV-CaMKIIα-ChR2-eYFPAAV-CMV-flex-shVGAT-eGFPAAV-CMV-flex-shVGLUT-eGFP 注射部位:小鼠前额叶皮质 注射方法: 0.2—0.4 μL 检测时间:3 周 AAV-CaMKII...
病毒产品AAV9-CaMKII-eArchT-EYFPAAV9-EF1α-DIO-eArch3.0-EYFP 病毒滴度4×10E12 VG/mL4×10E12 ...
病毒方面,直接在目标脑区注射带有荧光蛋白载体的AAV,如AAV-hSyn-EYFP、AAV-DIO-ChR2-EYFP等等即可实现顺向示踪[5]。然而此种顺向示踪技术并不能排除路过某脑区而未形成突触这一现象,需后续电生理记录验证。 单纯疱疹病毒(HSV、 VSV)和伪狂犬病毒(PRV)都能实现跨突触的顺行追踪。2017年1月,美国南加州大学的李...
实际上,DMHBrs3→RPa神经元很少表达Gad2(2.6±0.5%),这是位于后弧的标记(尾至前囟-2.06 mm)。在髓质中,研究人员在孤束核和延髓头端腹外侧区中稀疏的GFP神经元。为了评估从视前区到DMHBrs3→RPa神经元的神经递质输入,研究人员进行了ChR2辅助电路映射。他们用AAV-ChR2-EYFP转染POA神经元,并使用注射在RPa中的...
AAV phSyn1-FSF-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA catalog : 65454 more info or order: Addgene product webpage citations: 1 Reference Madisen L, Garner A, Shimaoka D, Chuong A, Klapoetke N, Li L,et al. Transgenic mice for intersectional targeting of neural sensors and effectors with...
AAV9 [EF1a-eNpHR 3.0-EYFP],≥1E+13 GC/mL,100ul ￥2,580 24h OT036-100 AAV9 [EF1a-eArch 3.0-EYFP],≥1E+13 GC/mL,100ul ￥2,580 24h OT037-100 AAV9 [EF1a-ChR2-mCherry],≥1E+13 GC/mL,100ul ￥2,580 24h OT038-100
To test this, we injected a 1:1 mixture of AAV1-hSyn-Cre and a Cre-dependent ChR2 virus, AAV1-EF1α-DIO-ChR2-EYFP, into V1 of Ai14 mice (Figure 4B, left panel). Only neurons co-transduced with the two viruses could express ChR2. In slice preparations, we examined monosynaptic...
AAV-OT134 ssAAV.CaMKlla- DIO eNpHR 3.0- EYFP AAV-OT135 ssAAV.CaMKlla- eNpHR 3.0- EYFP AAV-OT137 ssAAV.CaMKlla- ChR2- mCherry GFAP AAV-OT171 ssAAV.GFAP-double floxed-EYFP AAV-OT174 ssAAV.GFAP-DIO eNpHR 3.0-EYFP AAV-OT175 ssAAV.GFAP-eNpHR 3.0-EYFP AAV-OT176 ssAAV.GFAP-eArch...
AAV9 [GFAP-DIO eNpHR 3.0-EYFP],≥1E+13 GC/mL,100ul ￥2,580 24h OT175-100 AAV9 [GFAP-eNpHR 3.0-EYFP]≥1E+13 GC/mL,100ul ￥2,580 24h OT176-100 AAV9 [GFAP-eArch 3.0-EYFP],≥1E+13 GC/mL,100ul ￥2,580 24h OT177-100 AAV9 [GFAP-ChR2-mCherry],≥1E+13 GC/mL,100ul...
sunbio AAV产品手册 最终版 上海生博AAV产品手册（2017版）一、AAV背景知识 1.1AAV概况 腺相关病毒(Adeno-associated virus，AAV)，是一类无包膜的细小病毒，属于微小病毒科(Parvoviridae)的依赖病毒属(Dependoparvovirus)，透射电镜(transmission electron microscope，TEM)下，AAV病毒本身呈二十面体结构。AAV 基因组...