①ChR2 由475 nm的蓝光激活，是阳离子选择性通道，允许阳离子（如Na+）大量内流，产生动作电位，使...
注射参数病毒类型: AAV-hSyn-HA-hM4Di-IRES-mCitrineAAV-CaMKIIα-eGFPAAV-hsyn-DIO-hM3Dq-eGFPAAV-hsyn-DIO-hM4Di-eGFPAAV-hsyn-DIO-mCherryAAV-CaMKIIα-ChR2-eYFPAAV-CMV-flex-shVGAT-eGFPAAV-CMV-flex-shVGLUT-eGFP 注射部位:小鼠前额叶皮质 注射方法: 0.2—0.4 μL 检测时间:3 周 AAV-CaMKII...
AAV phSyn1-FSF-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA catalog : 65454 more info or order: Addgene product webpage citations: 1 Reference Madisen L, Garner A, Shimaoka D, Chuong A, Klapoetke N, Li L,et al. Transgenic mice for intersectional targeting of neural sensors and effectors with...
AAV9 [CaMKIIa-eArch 3.0-EYFP],≥1E+13 GC/mL,100ul ￥2,580 24h OT137-100 AAV9 [CaMKIIa-ChR2-mCherry],≥1E+13 GC/mL,100ul ￥2,580 24h OT138-100 AAV9 [CaMKIIa-FLEX-ArchT-GFP],≥1E+13 GC/mL,100ul ￥2,580 24h
他们用AAV-ChR2-EYFP转染POA神经元,并使用注射在RPa中的HSV-lsl-mCherry识别DMHBrs3→RPa神经元。自此,研究人员发现记录的大多数DMHBrs3→RPa神经元接受来自POA的GABA能和谷氨酸能输入(图7)。这表明DMHBrs3→RPa电路受到POA的动态调控。 (图7) 结论
病毒方面,直接在目标脑区注射带有荧光蛋白载体的AAV,如AAV-hSyn-EYFP、AAV-DIO-ChR2-EYFP等等即可实现顺向示踪[5]。然而此种顺向示踪技术并不能排除路过某脑区而未形成突触这一现象,需后续电生理记录验证。 单纯疱疹病毒(HSV、 VSV)和伪狂犬病毒(PRV)都能实现跨突触的顺行追踪。2017年1月,美国南加州大学的李...
AAV9 [CaMKIIa-DIO eNpHR 3.0-EYFP],≥1E+13 GC/mL,100ul ￥2,580 24h OT135-100 AAV9 [CaMKIIa-eNpHR 3.0-EYFP],≥1E+13 GC/mL,100ul ￥2,580 24h OT136-100 AAV9 [CaMKIIa-eArch 3.0-EYFP],≥1E+13 GC/mL,100ul ￥2,580 24h OT137-100 AAV9 [CaMKIIa-ChR2-mCherry],≥1E+13 ...
AAV-mDLX-mCherry (AAV Serotype 2) >1x10^13 VG/mL 30 µL $168 ~ $488 SL116115 AAV-Syn-hChR2(H134R)-EYFP (Serotype 2) >1x10^13 VG/mL 30 µL $168 ~ $488 SL116303 AAV-EF1a-mCherry (Serotype 2) >1x10^13 VG/mL 30 µL $168 ~ $488 ...
AAV9 [CaMKIIa-DIO eNpHR 3.0-EYFP],≥1E+13 GC/mL,100ul ￥2,580 24h OT135-100 AAV9 [CaMKIIa-eNpHR 3.0-EYFP],≥1E+13 GC/mL,100ul ￥2,580 24h OT136-100 AAV9 [CaMKIIa-eArch 3.0-EYFP],≥1E+13 GC/mL,100ul ￥2,580 24h OT137-100 AAV9 [CaMKIIa-ChR2-mCherry],≥1E+13 ...
Description: AAV2-Syn-hChR2(H134R)-EYFP is a pre-packaged rAAV in serotype 2 (with capsid from AAV serotype 2 and 2xITR from AAV serotype 2) which over-expresses the humanized ChR2(H134R) fused to EYFP driven by the human synapsin promoter for optogenetic activation. ...