Reagents Antibodies against Bcl-2, cyclin A1, proliferating cell nuclear antigen (PCNA) and retinoblastoma (Rb) protein were purchased from Santa Cruz Biotechnology (California, USA). Anti-HO-1 and anti-HO-2 were from Stressgen (Exeter, UK). Anti-phospho RbThr821and anti-vimentin were from I...
The monoclonal antibody specific to integrinα6 (ITGA6), ITGB4, LAMC2, or FABP5, and polyclonal antibody against CAV1, calreticulin or TIP47 were purchased from Santa Cruz (Santa Cruz, CA). The monoclonal antibody specific to PFN2, and polyclonal antibody against annexin A3 were purchased ...
In order to shed more light on the specificity of SLC44A1-PRKCA fusion and its potential value in the dif- ferential diagnosis of PGNT, we also investigated thirteen additional cases of PGNT mimics. Particularly, we studied five RGNTs cases that could be a differential diagnosis due to the...
2). In NEPC, the FOXA1 promoter shed contacts with its regulatory region identified in PRAD15 and looped to a distinct NEPC-restricted super-enhancer (Fig. 2d). Both the distal super-enhancer and promoter were co-bound by FOXA1 and ASCL1, suggesting an auto-regulatory circuit that is ...
After formaldehyde fixation, lysed cell DNA was sheared by sonication and immunoprecipitated with an anti-NANOG antibody (H-155, cat# sc-33759; Santa Cruz Biotechnology, Santa Cruz, CA, USA) and protein-A beads overnight. DNA was eluted from the beads and prepared for sequencing. Sequenced ...
catastrophe. MRE11 inhibition had no effect on PCNA/RPA1 dynamics regardless of ATR activity. E3 ligase RAD18 was recruited at stalled replication forks in parallel to PCNA removal. Our results shed light on fork dynamics during nucleotide depletion and provide a valuable tool for interrogating ...
Our results shed light on fork dynamics during nucleotide depletion and provide a valuable tool for interrogating the effects of replication stress-inducing anti-cancer agents. Keywords: replication stress; fork stalling; fork restart; PCNA; RPA; hydroxyurea; RAD18...
On one hand EVs may program the immune system to elicit an anti-tumour response, on the other hand they could function as a long-distance communication tool by which tumour cells may form pre-metastatic niches in secondary organs [15]. Thus, in the last decade, EVs have been considered ...