2). In NEPC, the FOXA1 promoter shed contacts with its regulatory region identified in PRAD15 and looped to a distinct NEPC-restricted super-enhancer (Fig. 2d). Both the distal super-enhancer and promoter were co-bound by FOXA1 and ASCL1, suggesting an auto-regulatory circuit that is ...
Accordingly, delineating the propensity of PRA would shed light on the nature of PRA1-LMP1 interaction and yield additional insights into the tumorigenesis of NPC. To further assess the role of PRA1 in NPC cells, in this study we generated several PRA1-knockdown NPC cell clones, which ...
Reagents Antibodies against Bcl-2, cyclin A1, proliferating cell nuclear antigen (PCNA) and retinoblastoma (Rb) protein were purchased from Santa Cruz Biotechnology (California, USA). Anti-HO-1 and anti-HO-2 were from Stressgen (Exeter, UK). Anti-phospho RbThr821and anti-vimentin were from I...
antibody (Novus Biologicals, NBP1-28566); anti-Bcl-2 anti- body (Santa Cruz, sc-7382); anti-cleaved caspase-3 (Asp175) (Cell Signaling Technology, 9661); anti-ALDH 1A1 antibody (Abcam, EP1933Y); anti-β-Actin (Abcam, ab14128); anti-rabbit IgG, HRP-linked antibody (Cell Signaling ...
Prostate cancer is the most common cancer in men in the Western world and the number is rising. Prostate cancer is notoriously heterogeneous, which makes it hard to generate and study in pre-clinical models. The family of Forkhead box (FOX) transcription
On one hand EVs may program the immune system to elicit an anti-tumour response, on the other hand they could function as a long-distance communication tool by which tumour cells may form pre-metastatic niches in secondary organs [15]. Thus, in the last decade, EVs have been considered ...
In the same issue of Nature, Stan Woosley of the University of California at Santa Cruz and his colleagues show how multiple explosions in a single, very massive star could account for 2006gy’s behaviour. J. In this model, every explosion produces an expanding shell of material. When new...
After formaldehyde fixation, lysed cell DNA was sheared by sonication and immunoprecipitated with an anti-NANOG antibody (H-155, cat# sc-33759; Santa Cruz Biotechnology, Santa Cruz, CA, USA) and protein-A beads overnight. DNA was eluted from the beads and prepared for sequencing. Sequenced ...
Optic atrophy 1 (OPA1) is a mitochondrial inner membrane protein that has an important role in mitochondrial fusion and structural integrity. Dysfunctional OPA1 mutations cause atrophy of the optic nerve leading to blindness. Here, we show that OPA1 has