The E. coli strain BL21 (DE3) for the heterologous expression of the P450s was purchased from Agilent Technologies (Santa Clara, CA, USA). Bioinformatics. Pairwise protein sequence alignments were performed using the Needlemann-Wunsch algorithm (EMBL-EBI: Needle (EMBOSS)). Protein sequences...
The next challenge is to identify the target genes acting downstream of HO-1 and its products and ultimately to determine whether these pathways might be targeted therapeutically. Analysis of HO-1 enzymatic product CO has shed some light and suggests CO may contribute to the proliferative and pro...
The amplified products were studied by direct sequencing after clean-up exonuclease ExoSAP-IT (Affymetrix, Santa Clara, CA) using the Big Dye Terminator Cycle Sequencing Kit and capillary electrophoresis on the automated sequencer ABI3730 (Applied Biosystems, Carlsbad, CA). Sense and antisense se- ...
pub- lished the comparison of microRNA expression patterns from fresh-frozen and corresponding FFPE lymph node specimens (n = 7) using the Agilent microarray platform (Agilent, Santa Clara, CA). [14] These authors also found a very good correlation between measurements of matched fresh-frozen ...
The complex and dynamic three-dimensional organization of chromatin within the nucleus makes understanding the control of gene expression challenging, but also opens up possible ways to epigenetically modulate gene expression. Because plants are sessile,
Article https://doi.org/10.1038/s41467-023-36227-3 HSFA1a modulates plant heat stress respon- ses and alters the 3D chromatin organization of enhancer-promoter interactions Received: 29 June 2022 Accepted: 19 January 2023 Check for updates Ying Huang 1, Jing An 1, Sanchari Sircar1, Clara ...