Directed mutation is a proposed process that allows mutations to occur at higher frequencies when they are beneficial. Until now, the existence of such a process has been controversial. Here we describe a novel mechanism of directed mutation mediated by the transposon, IS 5 in Escherichia coli ....
aThe presence of the transposon insertion in eight representative two-line male sterile lines. M, DL2000 Plus DNA Marker. To detect the transposon insertion, we used forward (F) and reverse (R) primers located downstream of the transposon gene and upstream ofUBC12, respectively; the PCR produ...
A. et al. Compound inheritance of a low-frequency regulatory SNP and a rare null mutation in exon-junction complex subunit RBM8A causes TAR syndrome. Nat. Genet. 44, 435–439 (2012). The first study to link mutations in Y14 to a human disease. In this case, Y14 expression is ...
As well as investigating changes in expression on the gene level, we investigated changes on the transcript level via differential exon usage (DEU) analysis, as changes in exon/transcript usage don’t always result in differential expression on the gene level. Supertranscripts, containing all unique...
Compound inheritance of a low-frequency regulatory SNP and a rare null mutation in exon-junction complex subunit RBM8A causes TAR syndrome. Nat Genet. 2012;44:435–9. Article CAS PubMed Central PubMed Google Scholar Kurotaki N, Shen JJ, Touyama M, Kondoh T, Visser R, Ozaki T, et al...
The Tol2kit: A multisite gateway-based construction kit for Tol2 transposon transgenesis constructs. Dev. Dyn. 236, 3088- 3099. https://doi.org/10.1002/dvdy.21343 Google Scholar Crossref PubMed Lewis, T. R., Zareba, M., Link, B. A. and Besharse, J. C. ( 2018). ...
In contrast, he pointed to evidence consistent with the silencing of transposons by DNA methylation in somatic cells, where transposon activity would be more detrimental than in germ and stem cells [112]. Why, Bird asked, would an organism fail to protect its germline from these parasitic DNA ...
However, an examination of the Repeatmasker tables associated with the white rhinoceros genome did not reveal any type of transposon that amplified at the time of L1's demise. Finally, the demography of a host can potentially affect the rate of fixation of insertions. When an organism has a ...
De novo mutations are known to play a prominent role in sporadic disorders with reduced fitness. We hypothesize that de novo mutations play an important role in severe male infertility and explain a portion of the genetic causes of this understudied diso
In several species, Piwi/piRNA genome silencing defects cause immediate sterility that correlates with transposon expression and transposon-induced genomic instability. In C. elegans, mutations in the Piwi-related gene (prg-1) and other piRNA deficient m