Modifications of RNA affect its function and stability. RNA editing is unique among these modifications because it not only alters the cellular fate of RNA molecules but also alters their sequence relative to the genome. The most common type of RNA editi
i，次黄嘌呤，腺嘌呤脱氨产物，是鸟嘌呤结构类似物，因此在复制、翻译等过程中，从a到i会导致碱基突变效果
作者使用了牛津纳米孔直接对RNA测序,用于鉴定天然转录组中含肌苷的位点,提出了Dinopore (Detection of inosine with nanopore sequencing)。作者训练了卷积神经网络模型估计每个编辑站点的修改率来区分是否存在RNA编辑位点。作者证明了它们在人类、小鼠和非洲爪蟾的转录组上的良好性能。作者的方法可进一步扩展到研究其他RNA修...
Adenosine-to-inosine (A-to-I) RNA editing is a post-transcriptional processing event involved in diversifying the transcriptome responsible for various biological processes. Although bioinformatic approaches have predicted a number of A-to-I editing sites in cDNAs, the human transcriptome is thought to...
A-to-I RNA editing Adenosine deaminases acting on RNA (ADARs) enzymes are responsible for catalyzing A-to-I editing in double-stranded RNA (dsRNA). Three ADAR genes—ADAR1, ADAR2, and ADAR3—have been identified in the human genome, each containing two to three N-terminal dsRNA-binding ...
▲ 而更为广泛存在的一类RNA编辑是在ADAR蛋白(adenosine deaminase acting on RNA)的作用下发生的A-to-I[11]。I(肌苷,也称次黄嘌呤核苷)偏好于与C配对,因此在功能上相当与G(A-to-G)。A-to-I编辑大多是位点特异性的(site-directed editing),但是在重复序列(如Alu原件)或病毒基因组中由于很多A的富集,也会...
Through an integrated analysis of TCGA genomic data and CPTAC proteomic data, our study provides large-scale direct evidence that A-to-I RNA editing is a source of proteomic diversity in cancer cells. Thus, RNA editing represents an exciting paradigm for understanding the molecular basis of human...
Adenosine-to-inosine (A-to-I) RNA editing is an epigenetic modification catalyzed by adenosine deaminases acting on RNA (ADARs), and is especially prevalent in the brain. We used the highly accurate microfluidics-based multiplex PCR sequencing (mmPCR-seq
A-to-I RNA editing occurs ubiquitously but most extensively in the central nervous system (CNS), and more than three million editing sites have been detected in humans (Picardi et al., 2015). The extent of the RNA editing varies among the different A-to-I sites and among the same A-...