This leads to a flattening of the funnel for DM-MBP in the region where the hydrophobic interactions in WT-MBP further guide folding of the NTD (Fig. 5e). Once the correct contacts are formed in the NTD, the N-C interface and finally CTD fold. In the presence of GroEL/ES, the ...
Using structural bioinformatics, we show that a universal mutational propensity drives sites that are buried in multimeric interfaces to accumulate hydrophobic substitutions to levels that are not tolerated in monomers. In a database of hundreds of families of multimers, most show signatures of long-...
To examine the effects of restoring fetal states to adult epicardium, we predicted paracrine interactions from the epicardium using CellPhoneDB. First, we found more communication from fetal epicardium in comparison with adult epicardium when interacting with other cells of the adult heart. By restoring...
The positive values of ΔH and ΔS suggested that noncovalent molecular interactions, such as hydrogen bonds and hydrophobic interactions, performed a dominant role in the self-assembly of the cellobiose/SPc complex. To further investigate the complexation of cellobiose/SPc complex with dsRNA, ...
This biochemical characterization thus revealed that base pair stability and limited structural distortion are not the only key elements to be considered for the enzymatic construction of metal UBPs [42]. Particularly, size-complementarity, π-stacking, and interactions with residues of the active ...
The ability of the hydrophobic clefts of CaMN and CaMC to form more favorable interactions with a Phe versus Cys may cause the functional domain in each CaM knockout mutant to compete for the same sequence in the FGF12A CaMBD, as was previously observed in the binding of (Ca2+)4-CaM ...
Moreover, sodium-dependent repetitive firing in Drosophila neurons is regulated by TipE [12], and Para-TipE interactions influence the pharmacology of the Para NaV channel [13]. Despite the lack of insect orthologues of vertebrate NaV channel auxiliary beta-subunits, characterization of the D. ...
It is well-known [35] that hydrophobic interactions are decisive factors in protein folding. Thus, we tested the power of the AHP we had generated to select correct protein folds exclusively on the basis of hydration energy, using a decoy selectivity test. We used Decoys'R'Us database [36...
(UGA)by the introduction of the additional anticodon stem base-pair G27A:U43A(yellow spheres).dDetails of the D-loop-T-loop tertiary interactions forming the elbow region in mtRNASer(UGA). The T-loop of mature human mtRNASer(UGA)contains modifications in positions U54 (m5U), U55 (ψ)...
hydrophobic residues as a steric gate to detect if there are any lesions at the minor groove19,20. Indeed, we previously reported the Pro-gate loop that interacts with incoming NTP and template base via Van der Waals interactions19,20. To examine the potential minor groove interactions between...