The spliceosome consists of small nuclear RNA proteins (snRNPs) U1, U2, U4, U5 and U6, together with approximately 80 conserved proteins. U5 snRNP contains nine specific proteins. This gene encodes one of the U5 snRNP-specific proteins. This protein belongs to the DEXH-box family of putative...
Each snRNP consists of one or two small nuclear RNAs (U1, U2, U4/U6, and U5 snRNAs) bound to both a set of proteins common to all snRNPs and a set of proteins particular to each snRNP (17). The common proteins, the Sm proteins (SmB/B′, SmD1, SmD2, SmD3, SmE, SmF, and ...
Ribonuclease P is an enzyme that cleaves tRNA precursors to generate the 5’ termini of mature tRNA sequences. In E. coli this enzyme consists of a catalytic RNA subunit and a protein cofactor. In the absence of direct cloning of the gene for the RNA com
Pre-mRNA splicing generates mature mRNAs from nascent transcripts and is catalyzed by thespliceosome, a dynamic macromolecular machine that consists ofsmall nuclear RNAs(snRNAs) and auxiliary proteins. Five snRNPs (U1, U2, U4, U5, and U6) constitute theribonucleoproteinsubunits of thespliceosome, the...
The structure of this body was visualizad by several different staining procedures: conventional electron microscopic techniques, a silver reaction, and the regressive EDTA staining for ribonucleoproteins. The silver-impregnation method employed here, which consists of a silver development sequence on ...
SIP1 is one of the proteins found in the SMN complex, which consists of the survival of motor neuron (SMN) protein and several gemin proteins. The SMN complex is localized to a subnuclear compartment called gems (gemini of coiled bodies) and is required for assembly of spliceosomal snRNPs ...
Functional interaction of a novel 15.5kD[U4/U6·U5] tri-snRNP protein with the5′ stem–loop of U4 snRNA site on the U4 snRNA consists of an internal purine-rich loop flanked by the stem of the 5′ stem–loop and a stem comprising two base pairs. Addit... S Nottrott,K Hartmuth,P...
A large, macromolecular complex composed of small nuclear RNAs and protein factors that removes regions (mostly introns) from transcribed precursor mRNA. RNA-binding proteins (RBPs). Proteins that bind to RNA molecules through RNA-recognition motifs and exert nuclear and cytoplasmic post-transcriptional...
As Sm proteins can form stable interactions with many uridine-rich RNAs, the role of the SMN complex is to promote the specific interactions between Sm proteins and snRNAs (Pellizzoni et al., 2002, Raker et al., 1999). The SMN complex consists of Gemin proteins 2–8 and Unrip (Caris...
For example, Sm proteins, (components of the small nuclear ribonucleoprotein (snRNP) spliceosome subunits), showed faster turnover of ∼18 h in the cytoplasm, where snRNP proteins are assembled on snRNAs, compared with an average of ∼35 h in the nucleus, where the snRNPs function to ...