Defining the cellular response to pharmacological agents is critical for understanding the mechanism of action of small molecule perturbagens. Here, we developed a 96-well-plate-based high-throughput screening infrastructure for quantitative proteomics and profiled 875 compounds in a human cancer cell ...
ProTargetMiner as a proteome signature library of anticancer molecules for functional discovery Article Open access 16 December 2019 A proteome-wide atlas of drug mechanism of action Article 02 January 2023 Decrypting the molecular basis of cellular drug phenotypes by dose-resolved expression proteomi...
(B) Drug target annotation of our GBM library, in comparison to recent studies of cancer cell panels. Mechanism of action (MOA), disease indication, and target from the Drug Repurposing Hub database. (C) Two-way clustering of primary GBM cultures (rows) and drugs (columns) based on area...
Splicing is a complex cellular mechanism by which the immature or precursor RNA is processed, removing the sequences that will not be part of the final RNA, or introns, and binding together the exons that form the mature RNA [13,14]. However, most of the genes (> 95%) do not under...
More broadly, non-SCN metastases have higher expression of SCN-associated transcription factors than non-SCN primary tumors. Drug sensitivity and gene dependency screens demonstrate that these convergent SCNCs have shared vulnerabilities. These common vulnerabilities are found across unannotated SCN-like ...
highest expression at E16. We believe that TBXT plays an important role in E16; thus, we chose E16 as the focus of this study. This comprehensive single-cell map reveals previously unrecognized signaling pathways that will improve our understanding of the mechanism of short-tailed sheep formation...
Psychiatric disorder is one of the major public health challenges worldwide, ranking as the second most significant cause of premature death and disability [6]. Psychiatric disorders, due to their high prevalence, are the major causes of significant economic burden [7]. The pathophysiological mechan...
Antibodies for profiling the human proteome--The Human Pro- tein Atlas as a resource for cancer research. Proteomics 2012;12:2067-2077.Asplund A., Edqvist P.H., Schwenk J.M., Ponten F. Antibodies for profiling the human proteome-the human protein atlas as a resource for cancer research....
Surely the fact that different methodologies are used is a good thing. Even if the science has to move forward from the mere presence of irisin to a quantitative and genetically controlled empiricism, and to establishing mechanism. Reply
Hopkins, D.Phil, FRSC, FSB, Division of Biological Chemistry and Drug Design, College of Life Sciences, University of Dundee Plenary Session: The Life Sciences CIO Panel From managing big data and cloud computing capabilities to building virtual communities and optimizing drug development, the life...