Maxam AM, Gilbert W (1977) A new method for sequencing DNA. Proc Natl Acad Sci USA 74:560-564A new method for sequencing DNA. A M Maxam,W Gilbert. Proceedings of the National Academy of Sciences of the United States of America . 1977...
In this method, a fragment of DNA carrying the origin of replication from a single-stranded phage, F1 or M13, is inserted into a plasmid to make a single-stranded:double-stranded bifunctional plasmid of the shortened fragments into sequencing vectors. The new family of bifunctional plasmid ...
DNA sequencing techniques have evolved rapidly in the last 5 years by the introduction of new sequencing machines, denominated second-generation sequencers, next-generation sequencers or massive parallel sequencers. These technologies make it possible to determine the complete sequence of the human genom...
aDNA序列分析的另外一钟方法是发展高速平行操作工艺。例如,Sydney Brenner首创了一种方法,他们能够同时测定一百万条基因的序列。 The DNA sequence analysis other clock method is the development high speed parallel operation craft.For example, Sydney Brenner originated one method, they can simultaneously determin...
Cancer is a disease largely caused by genomic aberrations. Utilizing many rapidly emerging sequencing technologies, researchers have studied cancer genomes to understand the molecular statuses of cancer cells and to reveal their vulnerabilities, such as
Single-cell sequencing enables us to better understand genetic diseases, such as cancer or autoimmune disorders, which are often affected by changes in rare cells. Currently, no existing software is aimed at identifying single nucleotide variations or mi
The key feature of the new method, named RNA Capture Long Seq (CLS), is that it focuses specifically on the non-coding regions of thegenome, that are amplified and analysed using the most advanced sequencing. "In this way we could produce a detailed map of 3,500 long non-coding RNAs ...
Here, we describe a method to detect copy number variation using shotgun sequencing, CNV-seq. The method is based on a robust statistical model that describes the complete analysis procedure and allows the computation of essential confidence values for detection of CNV. Our results show that the...
Targeted high-throughput DNA sequencing is a primary approach for genomics and molecular diagnostics, and more recently as a readout for DNA information storage. Oligonucleotide probes used to enrich gene loci of interest have different hybridization kinetics, resulting in non-uniform coverage that incre...
SEQC - Single-Cell Sequencing Quality Control and Processing Software, a general purpose method to build a count matrix from single cell sequencing reads, able to process data from inDrop, drop-seq, 10X, and Mars-Seq2 technologies. Paper Azizi, Elham, Ambrose J. Carr, George Plitas, Andre...