背景资料:Foregene One-Step RT-PCR Easy系列产品实现了从 RNA 到双链 DNA 的一体化反应,即逆转录和PCR扩增在同一个反应离心管、 同一个反应体系中完成, 简化了实验步骤、提高了实验效率、优化了实验方案。 产品描述:RT-PCR EasyTMII (Two Step) 包含两步法 RT-PCR 的全部试剂,采用 Foregene 公司独特的 RT...
酵素特性:產品內含AMV reverse transcriptase及Hot-start PCR mix,高效率、高專一性,可於50℃下作用合成DNA (效率較M-MLV佳) 引子種類:產品內含Oligo (dT)18及random hexamer primer供您使用 (亦可使用自行設計的專一性引子) 應用範圍:2ndcDNA synthesis, PCR, cloning等 Figure 1. RT-PCR products of E.coli ...
A three-step PCR that utilizes flexible primer choices to construct the library for Illumina amplicon sequencing has been applied to several studies in forest and agricultural systems. The three-step PCR protocol, while producing high-quality reads, often yields a large number (up to 46%) of ...
Methods A high-throughput real-time PCR system with an new designed integrated fluidic circuit assay were performed AMR gene detection. A total of 273 broiler fecal samples collected from two geographically separated farms were screened AMR genes. Results The new assay with limits of detection rangin...
A sensitive assay based on HPV-oncogene transcripts which are highly specific for cervical cancer cells was established. The Digital-Direct-RT-PCR (DD-RT-PCR) combines Ficoll-separation, ThinPrep-fixation and one-step RT-PCR in a low-throughput digital-PCR format enabling the direct analysis ...
Improved sensitivity of HBV-DNA tests is of critical importance for the management of HBV infection. Our aim was to develop and assess a new ultra sensitive in-house real-time PCR assay for HBV-DNA quantification (ultra sensitive RTQ-PCR). Previously use
Reactions were diluted two- to fivefold with H2O, and qPCR was performed using PowerUp SYBR Green PCR Master mix (Thermo Fisher Scientific), 2 μl diluted cDNA preparation and 0.4 μM of primers using a QuantStudio 6 Real-Time PCR system (Thermo Fisher Scientific). All qPCR primers...
(Vazyme) according to the manufacturer’s instructions. RT-qPCR was performed using Hieff® qPCR SYBR Green Master Mix (No ROX, Yeasen, China) and specific primers (TableS4) in an iQ5™ quantitative PCR system (Bio-Rad, USA). Expression levels were calculated using the 2 − Δ...
Mitochondrial DNA (mtDNA) encodes essential subunits of the oxidative phosphorylation system, but is also a major damage-associated molecular pattern (DAMP) that engages innate immune sensors when released into the cytoplasm, outside of cells or into circulation. As a DAMP, mtDNA not only contribute...
PCR products. The primer pairs and template DNA used for PCR are indicated for each lane, and the target bands are marked with an arrow. M, DNA size marker. Image of representative agarose gel from two independent experiments are shown.cExcision rates of SP1 inS. psychrophilaWP2 under ...