本制品是2 Step Real Time RT-PCR用的理想反转录反应试剂。5×PrimeScript RT Master Mix中含有定量RT-PCR的反转录反应所需的所有试剂 (PrimeScript RTase、RNase Inhibitor、Random 6 mers、Oligo dT Primer、dNTP Mixture、反应Buffer),加入模板RNA和水即可迅速进行反应。使用具有较强延伸能力的PrimeScript RTase,与...
本制品是采用probe法进行Real-time one-step RT-PCR反应的专用试剂。Real-time PCR方法以其*的快速性及定量性被广泛应用于科研领域,使用本制品进行Real-time RT-PCR反应可在同一反应管内连续进行,操作简单,并能有效防止污染。本反应体系由于可以对扩增产物进行实时检测,大大提高了检测灵敏度,并省略了PCR反应后的...
而NTC是指无模板对照——即用水代替荧光定量PCR 反应中的核酸。其他的试剂按比例正常加入,用于监测反应体系中的污染。正常情况下,NTC 孔中不会有扩增。当NTC 孔出现扩增时,则预示体系中可能有污染。 (扩增曲线:图来自real-time PCR handbook from ThermoFisher) 那...
结论 经 一 步法Real—timeRT—PCR检测,Gal—PEG—PEI/psiRNA纳米粒对HepG2细胞HLA—E基因有较高的沉默效率。 【关键词】 Real—timeRT—PCR;RNA干扰;人类白细胞抗原一E Detection ofinterferingefficiencyofHLA ——EmRNA expressioninHepG2cellusingone——stepre-· altimeRT—PCRmethod NIEChang-fu,HANFeng...
Real-time RT-PCR quantification of insulin-like growth factor (IGF)-1, IGF-1 receptor, IGF-2, IGF-2 receptor, insulin receptor, growth hormone receptor, IG... Pfaffl, MW, et al.Real-time RT-PCR quantification of insulin-like growth factor (IGF)-1, IGF-1 receptor, IGF-2, IGF-2 ...
One of the technological solutions of the genomic age is the fluorescence-based, real-time, quantitative reverse transcription polymerase chain reaction (RT-PCR), which has become the preferred method for mRNA detection. A number of factors have aided the adaptation of this technology into a mainst...
the real-time PCR was performed using an Applied Biosystems Step Two Real-Time PCR System (Applied Biosystems) and using the comparative Ct quantization method and compared with internal control. GAPDH was used as an internal control. Three biological replicates were used for analysis, and all ...
Real-time RT-PCR quantifi- cation of insulin-like growth factor IGF-1, IGF-1 receptor, IGF-2, IGF-2 receptor, insulin receptor, growth hormone receptor, IGF- binding proteins 1, 2 and 3 in the bovine species. Domest. Anim. Endocrinol. 22, 91-102....
2-step Real-time RT-PCR2-step Real-time RT-PCR的逆转录引物选择的逆转录引物选择Amplicon 3-end: 2 kb(N)9 Oligo-dT+(N)9Oligo-dTAmplicon 14、3-end: 6 kbOligo-dT Oligo-dT+(N)9(N)9(A)n运用Oligo-dT作为引物:扩增离3端远近不同的片段,离3越远扩增越差必需运用Oligo-dT+(N)9附录附录...
step real time RT- PCR.%目的 应用一步法Real - time RT - PCR检测不同载体转染HepG2细胞沉默人类白细胞抗原-E (HLA -E)基因的效率.方法 分别用Gal - PEG - PEI/psiRNA、Gal - PEG - PEI/NC - psiRNA、PEG - PEI/psiRNA、Lipofectamine 2000/psiRNA、裸psiRNA转染HepG2细胞,提取细...