Per well, 20 μl of the antibody cocktail was added and incubated for 1 h at room temperature. For imaging, a PerkinElmer Opera Phenix automated spinning-disk confocal microscope was used and each well of a 348-well plate was imaged at 20× magnification with 5 × 5 non-...
Xiaoying Zhang, Signal Transduction and Targeted Therapy, 2022 Antibody and T cell responses against wild-type and Omicron SARS-CoV-2 after third-dose BNT162b2 in adolescents Xiaofeng Mu, Signal Transduction and Targeted Therapy, 2022 Meplazumab in hospitalized adults with severe COVID-19 (DEFLECT...
aUMAP graph visualizes type and clustering of innate immune cells.bViolin diagram showing the expression level of NK cell effector proteins in the control and BA.2-BTI-6m groups.Pvalues are calculated by the two tailed Wilcoxon rank sum test, ****p < 0.0001, nsp > 0.05.cVolcanic...
It is also possible that the αA-crystallin/EGFP fusion proteins precipitate with other proteins to interfere with antibody recognition consistent with a range of lens opacities found in all transgenic lenses studied (see Additional file 2). Even if the protein remains unblocked, a constant, but...
To address the importance of microbial and hepatic biotransformation in ASIV treatment for ICH. The ICH targets were screened out and intersected with the compound targets before and after transformation. As displayed in the Venn diagram, 64 ICH-related genes were also targeted by ASIV. After bi...
Donor-specific antibody Primary graft failure Allogeneic HSCT INTRODUCTION Hematopoietic stem cell transplantation (HSCT) is the treatment of choice for many patients with such conditions as hematologic malignancies, solid tumors, and several nonmalignant congenital conditions [1]. According to a 2018 repo...
Using Caenorhabditis elegans, we reveal here that a pair of mitochondrion-localized transporters controls the mitochondrial level of Zn2+. We demonstrate that SLC-30A9/ZnT9 is a mitochondrial Zn2+ exporter. Loss of SLC-30A9 leads to mitochondrial Zn2+ accumulation, which damages mitochondria, ...
Study Flow Diagram View LargeDownload Figure 2. Forest Plot for Studies of Incubation Period of COVID-19 Caused by Different Variants View LargeDownload Figure 3. Incubation Period for COVID-19 in Older Patients and Infected Children View LargeDownload Infectious strains were original strains. ...
Percentage of cells positive by antibody staining (bottom). Background staining of a cell population electroporated with a non-targeting control sgRNA indicated with a dashed horizontal line. n = 5 electroporation replicates per condition. Data are presented as mean values +/− SD. ...
To gain insights into how PKB regulates calcium reuptake into SR of cardiomyocytes, we used a generic antibody recognizing phospho-Akt substrates (named the PAS antibody) to identify possible PKB substrates in the heart as previously reported19. PAS-reactive signals were increased on a number of ...